Veterinary Hospital Dissemination of CTX-M-15 Extended-Spectrum Beta-Lactamase–ProducingEscherichia coliST410 in the United Kingdom

Autor: Iuliana E Maciuca, Andrew Wattret, Nicola J. Williams, Vanessa Schmidt, Dorina Timofte
Rok vydání: 2016
Předmět:
0301 basic medicine
Veterinary medicine
medicine.medical_treatment
Veterinary Microbiology
Cephalosporin
Antibiotics
Gene Expression
Cat Diseases
medicine.disease_cause
Plasmid
Genotype
Dog Diseases
Escherichia coli Infections
Cross Infection
Cephalosporin Resistance
Pets
veterinary
infection control
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
Phenotype
surveillance
Microbiology (medical)
medicine.drug_class
030106 microbiology
Immunology
Microbial Sensitivity Tests
Microbiology
beta-Lactamases
Hospitals
Animal
03 medical and health sciences
Dogs
Escherichia coli
medicine
Animals
Pharmacology
business.industry
E. coli
biochemical phenomena
metabolism
and nutrition
bacterial infections and mycoses
United Kingdom
Cephalosporins
Carriage
ESBL
Cats
Beta-lactamase
Multilocus sequence typing
business
Multilocus Sequence Typing
Zdroj: MICROBIAL DRUG RESISTANCE
Microbial Drug Resistance
ISSN: 1931-8448
1076-6294
DOI: 10.1089/mdr.2016.0036
Popis: We characterized extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) in 32 Escherichia coli extended spectrum cephalosporin (ESC)-resistant clinical isolates from UK companion animals from several clinics. In addition, to investigate the possible dissemination of ESBL clinical isolates within a veterinary hospital, two ESBL-producing E. coli isolates from a dog with septic peritonitis and a cluster of environmental ESC-resistant E. coli isolates obtained from the same clinic and during the same time period, as these two particular ESBL-positive clinical isolates, were also included in the study. Molecular characterization identified blaCTX-M to be the most prevalent gene in ESC-resistant isolates, where 66% and 27% of clinical isolates carried blaCTX-M-15 and blaCTX-M-14, respectively. The only PMQR gene detected was aac(6')-Ib-cr, being found in 34% of the ESC E. coli isolates and was associated with the carriage of blaCTX-M-15. The clinical and environmental isolates investigated for hospital dissemination had a common ESBL/AmpC phenotype, carried blaCTX-M-15, and co-harbored blaOXA-1, blaTEM-1, blaCMY-2, and aac(6')-Ib-cr. Multilocus sequence typing identified them all as ST410, while pulse-field gel electrophoresis demonstrated 100% homology of clinical and environmental isolates, suggesting hospital environmental dissemination of CTX-M-15–producing E. coli ST410.
Databáze: OpenAIRE
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