Modification of chlorosulfonated polystyrene substrates for bioanalytical applications
| Autor: | Valeri Pavlov, Nerea Briz, Beatriz Díez-Buitrago, F.J. Fernández-SanArgimiro, Jaione Lorenzo |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Surface Properties Bioengineering Enzyme-Linked Immunosorbent Assay 02 engineering and technology 010402 general chemistry 01 natural sciences Colorimetry (chemical method) Biomaterials Contact angle chemistry.chemical_compound Adsorption X-ray photoelectron spectroscopy medicine Humans Amines Serum Albumin chemistry.chemical_classification Alkene Photoelectron Spectroscopy 021001 nanoscience & nanotechnology Human serum albumin 0104 chemical sciences Chemical engineering chemistry Mechanics of Materials Covalent bond Polystyrenes Polystyrene Sulfonic Acids 0210 nano-technology Hydrophobic and Hydrophilic Interactions medicine.drug |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 112 |
| ISSN: | 1873-0191 |
| Popis: | In this work the modification of polystyrene micro-well plates and their use as bioanalytical platform is described. A wet-chemical procedure was applied for the chlorosulfonation of these polystyrene substrates (PS) resulting in well-controlled and reactive surfaces. This method enabled the production of transparent and stable substrates under ambient conditions. The chlorosulfonyl moieties at the substrate surface were converted under mild conditions into different functional groups. The modification of PS served to increase the hydrophilic properties of the surface and thus, the improvement of interaction with biocompounds. The resulting substrates were characterized by contact angle measurements, X-ray Photoelectron Spectroscopy and colorimetry. PS substrates modified with different functional groups and attachment approaches (covalent link and direct adsorption of the antibodies) were used as the platform for immunoassays and the results compared to a commercial Human Serum Albumin ELISA kit. Aminated surfaces gave better results than those with carboxyl, alkene or epoxy groups and even the commercial kit. |
| Databáze: | OpenAIRE |
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