Liquid chromatography-tandem MS/MS method for simultaneous quantification of paracetamol, chlorzoxazone and aceclofenac in human plasma: An application to a clinical pharmacokinetic study
| Autor: | Safaa Toubar, Marwa I. Helmy, Maha A. Hegazy, Mona S. Elshahed, Dalia Mohamed |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Male
Electrospray Diclofenac Coefficient of variation Clinical Biochemistry 010402 general chemistry Mass spectrometry 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Drug Discovery medicine Ammonium formate Humans Sample preparation Molecular Biology Acetaminophen Pharmacology Chromatography Chemistry 010401 analytical chemistry Reproducibility of Results General Medicine 0104 chemical sciences Chlorzoxazone Linear Models Aceclofenac medicine.drug Chromatography Liquid |
| Zdroj: | Biomedical chromatography : BMC. 32(7) |
| ISSN: | 1099-0801 |
| Popis: | A facile, fast and specific method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous quantitation of paracetamol, chlorzoxazone and aceclofenac in human plasma was developed and validated. Sample preparation was achieved by liquid-liquid extraction. The analysis was performed on a reversed-phase C18 HPLC column (5 μm, 4.6 × 50 mm) using acetonitrile-10 mM ammonium formate pH 3.0 (65:35, v/v) as the mobile phase where atrovastatin was used as an internal standard. A very small injection volume (3 μL) was applied and the run time was 2.0 min. The detection was carried out by electrospray positive and negative ionization mass spectrometry in the multiple-reaction monitoring mode. The developed method was capable of determining the analytes over the concentration ranges of 0.03-30.0, 0.015-15.00 and 0.15-15.00 μg/mL for paracetamol, chlorzoxazone and aceclofenac, respectively. Intraday and interday precisions (as coefficient of variation) were found to be ≤12.3% with an accuracy (as relative error) of ±5.0%. The method was successfully applied to a pharmacokinetic study of the three analytes after being orally administered to six healthy volunteers. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text