| Autor: |
Wenjie Xu, Qiuping Li, Hanyue Xue, Yanjin Fei, Xiaowen Cui, Min Cao, Xiaohui Xiong, Xiong Xiong, Ying Yang, Libin Wang |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Food chemistry. 382 |
| ISSN: |
1873-7072 |
| Popis: |
One major drawback to the traditional loop-mediated isothermal amplification (LAMP) detection methods is the increased likelihood of detecting false-positive signals derived from non-specific amplification. Molecular beacon (MB) is increasingly being used in many applications and the MB-LAMP assay has proved itself as a target-specific method. The present work selected skipjack tuna as a case study, and developed a novel MB-LAMP assay for rapid species authentication. Specifically, the optimal MB structure includes 13 nucleobases in the loop region (binding specifically to loop primer LF) and 5 nucleobases in the stem region. For the established MB-LAMP assay, in the presence of the amplicons, the MB probe LFP-1 hybridizes to its target and forms a double helix. The change in conformation separates the quencher from the fluorophore, thereby resulting in the fluorescence release. The novel MB-LAMP assay has proved its specificity and can detect as little as 0.5 pg of skipjack tuna DNA. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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