Chromosome Fragments have the Potential to Predict Hyperthermia-induced Radio-sensitization in Two Different Human Tumor Cell Lines
| Autor: | Judith W.J. Bergs, Rosemarie ten Cate, Jaap Haveman, Chris van Bree, Jan Paul Medema, Nicolaas A. P. Franken |
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| Přispěvatelé: | CCA -Cancer Center Amsterdam, Radiotherapy |
| Rok vydání: | 2008 |
| Předmět: |
Hyperthermia
Radiation Cell Survival Health Toxicology and Mutagenesis Cancer Chromosome Dose-Response Relationship Radiation Chromosomal translocation Hyperthermia Induced Biology medicine.disease Radiation Tolerance Molecular biology Chromosomes Cell culture Cell Line Tumor Premature chromosome condensation Colonic Neoplasms Carcinoma Squamous Cell medicine Carcinoma Feasibility Studies Humans Radiology Nuclear Medicine and imaging Radiosensitivity |
| Zdroj: | Journal of radiation research, 49(5), 465-472. Japan Radiation Research Society |
| ISSN: | 1349-9157 0449-3060 |
| DOI: | 10.1269/jrr.07133 |
| Popis: | Cellular radiosensitivity, assessed by loss of clonogenicity, has been shown to correlate with the number of radiation-induced chromosomal aberrations. Also an increased radiosensitivity by hyperthermia has been shown to correlate with an increase in chromosomal aberrations. Therefore, determination of the number of chromosomal aberrations might be used as an assay to predict the radiosensitivity of tumors pre-treated with hyperthermia at clinically relevant temperatures. The use of premature chromosome condensation combined with fluorescent in situ hybridisation (PCC-FISH) has been shown to be clinically applicable. Therefore, the use of chromosomal aberrations as determined with PCC-FISH for the prediction of hyperthermia-induced radio-sensitization in human tumor cells was investigated. Confluent cultures of SW-1573 (human lung carcinoma) and RKO (human colorectal carcinoma) cells were treated with 1 h 41 degrees C or 43 degrees C hyperthermia prior to gamma-irradiation. Clonogenic cell survival and induction of chromosomal aberrations (unrejoined chromosomal fragments and translocations), by PCC-FISH, were studied at 24 h after treatment. Pre-treatment with hyperthermia at 41 degrees C for 1 h enhanced the radiosensitivity of RKO cells but not of SW-1573 cells. Increasing the temperature to 43 degrees C for 1 h enhanced the radiosensitivity of SW-1573 cells. When radio-sensitization was observed, a significant increase in the number of unrejoined chromosomal fragments was found but the frequency of translocations was not increased. Hyperthermia-induced radio-sensitization is correlated with an increase in unrejoined chromosomal fragments. This suggests that determination of the number of chromosomal fragments after hyperthermia and radiation treatment might be used for the prediction of combined treatment response in cancer patients. |
| Databáze: | OpenAIRE |
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