Hepatoprotective effect of parthenolide in rat model of nonalcoholic fatty liver disease
| Autor: | Majid Bahabadi, Farjam Goudarzi, Iraj Khodadadi, Adel Mohammadalipour, Mohammad Hashemnia, Jamshid Karimi, Nasrin Sheikh, Ghasem Solgi |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Thiobarbituric acid medicine.medical_treatment Immunology Intraperitoneal injection Toxicology medicine.disease_cause Diet High-Fat Protective Agents digestive system Antioxidants 03 medical and health sciences chemistry.chemical_compound Non-alcoholic Fatty Liver Disease Internal medicine Nonalcoholic fatty liver disease medicine TBARS Immunology and Allergy Animals Parthenolide Rats Wistar Triglycerides Pharmacology biology Superoxide Dismutase Tumor Necrosis Factor-alpha NF-kappa B Alanine Transaminase General Medicine CYP2E1 medicine.disease Rats Disease Models Animal Oxidative Stress 030104 developmental biology Endocrinology chemistry Alanine transaminase Liver biology.protein Sesquiterpenes Oxidative stress |
| Zdroj: | Immunopharmacology and immunotoxicology. 39(4) |
| ISSN: | 1532-2513 |
| Popis: | The active ingredients of traditional medical herbs have been the focus of scientific interests.This study was designed to explore the mechanisms of actions of parthenolide on nonalcoholic fatty liver disease (NAFLD).Thirty-five male Wistar rats were fed high-fat diet (HFD) for eight weeks with or without an intraperitoneal injection of parthenolide to develop NAFLD. Liver triacylglycerol (TG), total antioxidant capacity (TAC), total oxidative status (TOS), thiobarbituric acid reactant substances (TBARs), total thiol groups and tumor necrosis factor alpha (TNF-α) and cytochrome P4502E1 (CYP2E1) levels as well as liver ALT, AST and catalase activities were determined. In addition, quantitative real-time PCR was performed to obtain hepatic gene expression levels of TNF-α, CYP2E1 and nuclear factor-κB (NF-κB).HFD caused a significant weight gain and increased liver TG content as well as alteration in ALT and AST activities, which were attenuated after administration of parthenoide (p .05). Weakened liver antioxidant system (TAC, total thiol groups and catalase activity) and increased oxidative stress markers (TBARs and TOS) were mainly ameliorated by parthenolide treatment (p .05). Increased hepatic TNF-α, NF-κB and CYP2E1 at the both gene expression and protein levels were found associated with necroinflammatory changes in histopathological observations and were abrogated almost completely after parthenolide treatment. Oxidative and inflammatory changes observed in HFD fed rats were indicative of NAFLD, which were suppressed with parthenolide treatment.Based on these results, parthenolide might be a candidate agent for preventing NAFLD due to its anti-inflammatory and anti-oxidative potency. |
| Databáze: | OpenAIRE |
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