Enhanced expression of transforming growth factor beta during megakaryoblastic differentiation of K562 leukemia cells
Autor: | Tomi P. Mäkelä, Leif C. Andersson, Päivi J. Koskinen, Kari Alitalo, Riitta Alitalo |
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Rok vydání: | 1988 |
Předmět: |
medicine.medical_specialty
Cellular differentiation medicine.medical_treatment Immunology Biochemistry 03 medical and health sciences 0302 clinical medicine Internal medicine medicine 030304 developmental biology 0303 health sciences biology Growth factor Myeloid leukemia Cell Biology Hematology Transforming growth factor beta Molecular biology 3. Good health Endocrinology Cell culture 030220 oncology & carcinogenesis biology.protein Platelet-derived growth factor receptor Transforming growth factor K562 cells |
Zdroj: | Blood. 71:899-906 |
ISSN: | 1528-0020 0006-4971 |
Popis: | Platelet alpha granules contain several growth factors such as the transforming growth factor beta (TGF-beta) that are released during blood clotting and are thought to participate in the repair of tissue injury; however, the site of synthesis of platelet TGF-beta has not been demonstrated. We studied TGF-beta expression during megakaryoblastic differentiation of the chronic myeloid leukemia cell line K562 in vitro. These cells have mainly erythroid characteristics but acquire several megakaryoblastic properties when treated with the phorbol diester 12–0-tetradecanoyl-13-phorbolacetate (TPA). During four subsequent days of megakaryoblastic differentiation the amount of the 2.5-kilobase (kb) TGF-beta mRNA increased about eightfold, and a novel 2.3-kb mRNA species was induced in the K562 cells. This occurred concomitantly with distinct induction patterns of platelet-derived growth factor A (PDGF-A) and c-sis (PDGF-B chain) RNAs and several platelet antigens. The expression of erythroid markers such as glycophorin A decreased. Culture media of TPA-differentiated K562 cells also contained TGF-beta polypeptides as shown by a sensitive radioreceptor assay and by immunoprecipitation after metabolic labeling of the cells. These polypeptides were not seen in culture media from dimethyl sulfoxide- or sodium butyrate-treated cells. Unlike in several other cells, exogenously added TGF-beta 1 or 2 affected neither TGF- beta nor PDGF RNA expression in K562 cells. |
Databáze: | OpenAIRE |
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