Structural basis of microRNA length variety
| Autor: | Piotr Kozlowski, Wlodzimierz J. Krzyzosiak, Krzysztof Sobczak, Edyta Koscianska, Jacek Krol, Wojciech J. Szlachcic, Julia Starega-Roslan |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Genetics
Ribonuclease III Small interfering RNA Lin-4 microRNA precursor biology Base Sequence Molecular Sequence Data Computational biology Cell Line RNA silencing MicroRNAs RNA editing Mutagenesis microRNA biology.protein RNA Precursors Gene silencing RNA Humans RNA Processing Post-Transcriptional Drosha Dicer |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | The biogenesis of human microRNAs (miRNAs) includes two RNA cleavage steps in which the activities of the RNases Drosha and Dicer are involved. miRNAs of diverse lengths are generated from different genes, and miRNAs that are heterogeneous in length are produced from a single miRNA gene. We determined the solution structures of many miRNA precursors and analysed the structural basis of miRNA length diversity using a new measure: the weighted average length of diced RNA (WALDI). We found that asymmetrical structural motifs present in precursor hairpins are primarily responsible for the length diversity of miRNAs generated by Dicer. High-resolution northern blots of miRNAs and their precursors revealed that both Dicer and Drosha cleavages of imperfect specificity contributed to the miRNA length heterogeneity. The relevance of these findings to the dynamics of the dicing complex, mRNA regulation by miRNA, RNA interference and miRNA technologies are discussed. |
| Databáze: | OpenAIRE |
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