Measuring serum total and free indoxyl sulfate and p-cresyl sulfate in chronic kidney disease using UPLC-MS/MS
| Autor: | Chia-Ni Lin, I-Wen Wu, Shu-Yu Peng, Yun-Fen Huang, Ya-Ching Huang, Hsiao-Chen Ning |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Analyte 030309 nutrition & dietetics Calibration curve Population Renal function lcsh:TX341-641 Sulfuric Acid Esters Kidney Function Tests 01 natural sciences Cresols Young Adult 03 medical and health sciences Tandem Mass Spectrometry medicine Humans Protein precipitation Centrifugation Renal Insufficiency Chronic education Chromatography High Pressure Liquid Pharmacology Detection limit 0303 health sciences education.field_of_study Chromatography Chemistry 010401 analytical chemistry lcsh:RM1-950 medicine.disease 0104 chemical sciences lcsh:Therapeutics. Pharmacology Female Indican lcsh:Nutrition. Foods and food supply Food Science Kidney disease |
| Zdroj: | Journal of Food and Drug Analysis, Vol 27, Iss 2, Pp 502-509 (2019) |
| ISSN: | 1021-9498 |
| Popis: | Chronic kidney disease (CKD) is a complex disorder that affects multiple organs and increases the risk of cardiovascular complications. CKD affects approximately 12% of the population in Taiwan. Loss of kidney function leads to accumulation of potentially toxic compounds such as indoxyl sulfate (IS) and p-cresyl sulfate (pCS), two protein-bound uremic solutes that can stimulate the progression of CKD. The aim of this study was to assess whether IS and pCS levels were correlated with CKD stage. We developed and validated a method for quantitating total and free IS and pCS in serum by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Serum samples were pretreated using protein precipitation with acetonitrile containing stable isotope-labeled IS and pCS as internal standards. After centrifugation, the supernatant was diluted and injected into a UPLC-MS/MS system. Analyte concentrations were calculated from the calibration curve and ion ratios between the analyte and the internal standard. The calibration curves were linear with a correlation coefficient of >0.999; the analytical measurement range was 0.05–5 mg/L. The limit of quantitation of this assay was 0.05 mg/L for both analytes. The reference interval was ≤0.05–1.15 mg/L for total-form IS, ≤0.05–5.33 mg/L for total-form pCS, ≤0.05 mg/L for free-form IS, and ≤0.12 mg/L for free-form pCS. A positive correlation was observed between analyte concentration and CKD stage. Our sensitive UPLC-MS/MS method for quantifying total and free-form IS and pCS in serum can be used to monitor the progression of CKD in clinical settings, identify patients at risk, and facilitate development of further therapies for this devastating disease. Keywords: Chronic kidney disease, Mass spectrometry, Uremic toxins |
| Databáze: | OpenAIRE |
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