Force-feedback high-speed atomic force microscope for studying large biological systems
| Autor: | Ryan D. Boehm, Byung I. Kim |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Cantilever
business.industry Chemistry Atomic force microscopy General Physics and Astronomy Nanotechnology Cell Biology Microscopy Atomic Force Frame rate Optics Structural Biology Deflection (engineering) Biofilms Escherichia coli Image Processing Computer-Assisted General Materials Science Magnetic force microscope business Haptic technology |
| Zdroj: | Micron. 43:1372-1379 |
| ISSN: | 0968-4328 |
| DOI: | 10.1016/j.micron.2012.04.005 |
| Popis: | We designed and developed a high-speed atomic force microscope (HSAFM) utilizing a force-feedback scheme for imaging large biological samples. The system collects three simultaneous images: a deflection image, a topographic image, and a force image. We demonstrated that this force-feedback HSAFM is capable of acquiring large topographic images of Escherichia coli biofilms at approximately one frame per second in air. We discuss how the self-actuating cantilever and the piezo tube follow those larger biological topographic features during the HSAFM imaging process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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