Anticoagulant properties and cytotoxic effect against HCT116 human colon cell line of sulfated glycosaminoglycans isolated from the Norway lobster ( Nephrops norvegicus ) shell
| Autor: | Nadhem Sayari, Mohamed Ben Mansour, Imen Graiet, Ali Bougatef, Ikram Ben Amor, Rafik Balti, Jalel Gargouri |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Electrophoresis
0301 basic medicine Colon 02 engineering and technology Thrombin time Hemolysis Dermatan sulfate Glycosaminoglycan 03 medical and health sciences chemistry.chemical_compound Sulfation Animal Shells Nephrops norvegicus Spectroscopy Fourier Transform Infrared medicine Animals Humans Cytotoxicity Cell Proliferation Glycosaminoglycans Pharmacology Cell Death biology medicine.diagnostic_test Chemistry Anticoagulants General Medicine Heparan sulfate HCT116 Cells 021001 nanoscience & nanotechnology biology.organism_classification Glucuronic acid Nephropidae 030104 developmental biology Biochemistry Prothrombin Time Cattle Partial Thromboplastin Time Spectrophotometry Ultraviolet 0210 nano-technology |
| Zdroj: | Biomedicine & Pharmacotherapy. 80:322-330 |
| ISSN: | 0753-3322 |
| DOI: | 10.1016/j.biopha.2016.03.027 |
| Popis: | Sulfated glycosaminoglycans (SGNL) were extracted for the first time from Norway lobster (Nephrops norvegicus) shell. The monosaccharide composition analysed by GC/MS revealed the presence of galacturonic acid, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine. The analysis of SGNL with acetate cellulose electrophoresis in Zn-acetate revealed the presence of heparan sulfate (HS) and dermatan sulfate (DS). SGNL were evaluated for their anticoagulant activities using activated partial thromboplastin time (aPTT), thrombin time (TT) and prothrombine time (PT) tests. After 21h incubation, HCT116 cell proliferation was inhibited (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect