Neurotrophin 3 rescues neuronal precursors from apoptosis and promotes neuronal differentiation in the embryonic metanephric kidney
| Autor: | Jaan Palgi, Lauri Saxén, Hannu Sariola, Kirsi Sainio, Mart Saarma, Alexander Karavanov |
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| Rok vydání: | 1995 |
| Předmět: |
Neurite
Molecular Sequence Data Morphogenesis Gene Expression Apoptosis Nerve Tissue Proteins Neurotrophin-3 Biology Kidney 03 medical and health sciences 0302 clinical medicine Neurotrophin 3 Neurotrophic factors Neurites Animals Nerve Growth Factors RNA Messenger 030304 developmental biology DNA Primers Brain-derived neurotrophic factor Neurons 0303 health sciences Multidisciplinary Base Sequence Brain-Derived Neurotrophic Factor Molecular biology 3. Good health Cell biology Rats Nerve growth factor nervous system Neural Crest biology.protein 030217 neurology & neurosurgery Morphogen Neurotrophin Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 92(24) |
| ISSN: | 0027-8424 |
| Popis: | We analyzed the developmental regulation and role of the neurotrophins during metanephric kidney morphogenesis. RNase protection assay revealed the presence of nerve growth factor, neurotrophin 3 (NT-3), and brain-derived neurotrophic factor mRNAs and the regulation of their expression during embryonic development of rat metanephros. NT-3 induced differentiation (neurite outgrowth) and survival (inhibition of apoptosis) of the neuronal precursors in cultured nephrogenic mesenchymes and neuronal differentiation in cultured whole kidneys, whereas NT-4/5, brain-derived neurotrophic factor, and nerve growth factor were without effect. The neurotrophins did not trigger tubular differentiation of isolated nephrogenic cells, which underwent apoptosis when cultured with or without the neurotrophins. NT-3 is thus an inducer of differentiation and a survival factor for renal neuronal cells, but none of the neurotrophins is a morphogen in kidney tubule induction. |
| Databáze: | OpenAIRE |
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