The 4-aminopyridine in vitro epilepsy model analyzed with a perforated multi-electrode array
| Autor: | Rhonda Dzakpasu, Stefano Vicini, Gholam K. Motamedi, Alfredo Gonzalez-Sulser, Jing Wang, Massimo Avoli |
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| Rok vydání: | 2011 |
| Předmět: |
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Local field potential AMPA receptor local field potentials In Vitro Techniques Bicuculline Hippocampus Receptors N-Methyl-D-Aspartate Article Piperazines gamma-Aminobutyric acid Mice Cellular and Molecular Neuroscience Glutamatergic Quinoxalines Potassium Channel Blockers medicine Animals GABA-A Receptor Antagonists 4-Aminopyridine Electrodes gamma-Aminobutyric Acid seizures 4-aminopyridine gaba hippocampus Pharmacology Epilepsy Chemistry Dentate gyrus Isoxazoles Somatosensory Cortex Microarray Analysis CA3 Region Hippocampal Mice Inbred C57BL Disease Models Animal nervous system NMDA receptor GABAergic Anticonvulsants Neuroscience Software medicine.drug |
| Zdroj: | Neuropharmacology. 60:1142-1153 |
| ISSN: | 0028-3908 |
| DOI: | 10.1016/j.neuropharm.2010.10.007 |
| Popis: | Epileptiform discharges recorded in the 4-aminopyridine (4-AP) in vitro epilepsy model are mediated by glutamatergic and GABAergic signaling. Using a 60-channel perforated multi-electrode array (pMEA) on corticohippocampal slices from 2 to 3 week old mice we recorded interictal- and ictal-like events. When glutamatergic transmission was blocked, interictal-like events no longer initiated in the hilus or CA3/CA1 pyramidal layers but originated from the dentate gyrus granule and molecular layers. Furthermore, frequencies of interictal-like events were reduced and durations were increased in these regions while cortical discharges were completely blocked. Following GABA(A) receptor blockade interictal-like events no longer propagated to the dentate gyrus while their frequency in CA3 increased; in addition, ictal-like cortical events became shorter while increasing in frequency. Lastly, drugs that affect tonic and synaptic GABAergic conductance modulated the frequency, duration, initiation and propagation of interictal-like events. These findings confirm and expand on previous studies indicating that multiple synaptic mechanisms contribute to synchronize neuronal network activity in forebrain structures. This article is part of a Special Issue entitled 'Trends in neuropharmacology: in memory of Erminio Costa'. |
| Databáze: | OpenAIRE |
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