BIOCOMPATIBILITY OF BRILLIANT BLUE G IN A RAT MODEL OF SUBRETINAL INJECTION
| Autor: | Akifumi Ueno, Yoshinobu Goto, Toshio Hisatomi, Toshiaki Kubota, Hiroshi Enaida, Tadahisa Kagimoto, Yasuaki Hata, Tatsuro Ishibashi, Yasutaka Mochizuki |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Indocyanine Green
Retinal degeneration medicine.medical_specialty medicine.medical_treatment Apoptosis Biocompatible Materials Vitrectomy Basement Membrane Retina Injections chemistry.chemical_compound Rats Inbred BN Ophthalmology In Situ Nick-End Labeling medicine Animals Coloring Agents Pigment Epithelium of Eye Staining and Labeling business.industry Benzenesulfonates Retinal Degeneration Retinal Detachment Retinal detachment Epiretinal Membrane Retinal Trypan Blue General Medicine medicine.disease eye diseases Rats Disease Models Animal chemistry Toxicity Trypan blue business Indocyanine green |
| Zdroj: | Retina. 27:499-504 |
| ISSN: | 0275-004X |
| Popis: | Purpose To evaluate the toxicity of brilliant blue G (BBG) compared with those of indocyanine green (ICG) and trypan blue (TB) in a rat model of subretinal injection. Methods Retinal detachment was produced by subretinal injection of the dyes. The biocompatibility of BBG (0.25 mg/mL) was evaluated over 2 months and 2 weeks by ophthalmic examinations. The eyes were enucleated and analyzed by light, fluorescence, as well as transmission electron microscopy. Apoptotic cell death was detected by TdT-dUTP terminal nick-end labeling. The results were compared with those for ICG (5 mg/mL) and TB (1 mg/mL). Results ICG caused retinal degeneration and retinal pigment epithelial (RPE) cell atrophy 2 weeks after subretinal injection. Apoptotic cell death was detected in the inner and outer nuclear layers and the RPE layer, especially the photoreceptors. TB caused less retinal degeneration, mainly in the area detached by the subretinal injection. BBG had no detectable toxic effects after 2 months and 2 weeks. Apoptotic cell death was detected in the ICG and TB groups, mainly in the photoreceptors. Conclusions Subretinal injection of the dyes caused retinal cell degeneration at lower concentrations than those reported for intravitreous injection. However, subretinal injection of BBG at 0.25 mg/mL appeared to provide satisfactory biocompatibility. |
| Databáze: | OpenAIRE |
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