Complement activation by malignant B cells from patients with chronic lymphocytic leukaemia (CLL)
| Autor: | H. V. Marquart, B. E. Christensen, S E Svehag, Kirsten Grønbæk, R. G. Q. Leslie |
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| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Male
Chronic lymphocytic leukemia Immunology chemical and pharmacologic phenomena Complement receptor Biology Mice Complement Receptor Type 1 immune system diseases hemic and lymphatic diseases medicine Immunology and Allergy Animals Humans Decay-accelerating factor Complement Activation B-Lymphocytes CD46 Complement C3 medicine.disease Leukemia Lymphocytic Chronic B-Cell Complement system Alternative complement pathway Female Receptors Complement 3d CD5 Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | SUMMARYIt has previously been reported that the expression of the complement receptors CRI (CD35) and CR2 (CD21) on malignant B cells in CLL is reduced compared with the expression on normal B cells, while deposition of complement C3 fragments, as a consequence of alternative pathway (AP) activation of complement, is observed on mononuclear cells from patients with B CLL. Following our demonstration that normal B cells are capable of activating the AP of complement in a CR2- dependent fashion, we have chosen to re-examine the complement-activating ability of B CLL cells in relation to their altered phenotype with respect to CR2 and the complement regulatory membrane proteins. CRI. Decay accelerating factor (DAF) (CD55) and membrane cofactor protein (MCP) (CD46). Flow cytometry was used to measure expression of complement receptors and regulatory proteins on CDS+ B cells from CLL patients, as well as the deposition of C3 fragments occurring both in vivo and after in vitro AP activation. We have confirmed the reduced expression of CRI and CR2 on CLL cells and have shown that AP activation in the presence of homologous, normal serum was reduced on B CLL cells compared with normal B cells. The degree of AP activation correlated directly with CR2 expression. In addition, we observed that CLL cells bear in vivo-deposited C3d, g, although at a significantly lower level than normal B cells. |
| Databáze: | OpenAIRE |
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