Isolation and characterization of 20 polymorphic microsatellites loci for Xenocypris davidi based on high-throughput sequencing
| Autor: | Yuan Julin, Li Ming, Aihuan Guo, Zhimin Gu, Lian Qingping |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Heterozygote Population Cyprinidae Population genetics Locus (genetics) Biology Genome DNA sequencing Gene flow 03 medical and health sciences 0302 clinical medicine Genetics Animals education Molecular Biology Alleles Genetic diversity education.field_of_study Polymorphism Genetic General Medicine 030104 developmental biology Genetic Loci 030220 oncology & carcinogenesis Microsatellite Microsatellite Repeats |
| Zdroj: | Molecular Biology Reports. 47:8305-8310 |
| ISSN: | 1573-4978 0301-4851 |
| DOI: | 10.1007/s11033-020-05834-4 |
| Popis: | Xenocypris davidi is one of the most economically important freshwater fish in China. However, few molecular markers have been reported for this species, impeding in-depth population genetic, dispersal, and gene flow studies. In the present study, a batch of novel polymorphic microsatellites from the genome of X. davidi were isolated and characterized using high-throughput sequencing. A total of 20 microsatellite markers were isolated. Analysis of 33 individuals revealed an average of 7.35 alleles per locus, ranging from 3 to 18. The observed and expected heterozygosities ranged from 0.3 to 1 and from 0.426 to 0.93, respectively. Only one tested locus significantly deviated from Hardy–Weinberg equilibrium. 18 microsatellite loci were highly polymorphic (PIC > 0.5). These newly isolated microsatellite markers would be useful to study the population genetics and stock management of X. davidi. |
| Databáze: | OpenAIRE |
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