Cell-free plasma DNA: a marker for apoptosis during hemodialysis
| Autor: | Manfred Eigner, Mathias M. Mueller, Karl M. Stuhlmeier, Johanna Atamaniuk, Alireza Karimi, Katharina Ruzicka |
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| Rok vydání: | 2006 |
| Předmět: |
Adult
Programmed cell death Clinical Biochemistry Population Apoptosis Biology Necrosis Annexin Renal Dialysis medicine Leukocytes Humans Annexin A5 education Cell damage Aged Fluorescent Dyes Gel electrophoresis Aged 80 and over Cell Nucleus education.field_of_study Biochemistry (medical) DNA Middle Aged medicine.disease Molecular biology Agarose gel electrophoresis Dactinomycin Biomarkers |
| Zdroj: | Clinical chemistry. 52(3) |
| ISSN: | 0009-9147 |
| Popis: | Background: We evaluated whether cell-free plasma DNA might be an appropriate marker for cell damage during hemodialysis (HD) and whether it correlated with annexin V expression and 7-amino-actinomycin D (7AAD) nuclear staining of blood leukocytes.Methods: Circulating DNA, annexin V, and 7AAD were measured in HD patients before HD, 20 min after start of HD, and after HD had ended. Healthy volunteers provided control measurements. Necrosis and apoptosis were monitored by gel electrophoresis.Results: Plasma DNA concentrations were not significantly different between controls and patients before HD. Circulating DNA increased significantly (P Conclusions: During HD, cell-free plasma DNA concentrations, annexin V expression, and 7AAD uptake in leukocytes increases. The increase in plasma DNA, appearing as ladders typical of apoptosis, and the 7AAD uptake in leukocytes demonstrate that the predominant portion of circulating DNA in HD patients originates from apoptotic leukocytes. |
| Databáze: | OpenAIRE |
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