Maternal Microchimerism in Cord Blood and Risk of Celiac Disease in Childhood
Autor: | Marte Katrine Viken, Ketil Størdal, Georgina L. Mortimer, German Tapia, Karl Mårild, Saranna Chipper-Keating, Jody Ye, Benjamin Thomas Gillard, Kathleen M Gillespie, Lars C. Stene, Benedicte A. Lie |
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Rok vydání: | 2020 |
Předmět: |
Disease
Human leukocyte antigen Chimerism Cohort Studies 03 medical and health sciences 0302 clinical medicine Pregnancy 030225 pediatrics medicine Humans Enteropathy Child Fetus business.industry Gastroenterology Infant Newborn Odds ratio medicine.disease Fetal Blood Celiac Disease Cord blood Case-Control Studies Child Preschool Pediatrics Perinatology and Child Health Cohort Immunology 030211 gastroenterology & hepatology Female business |
Zdroj: | Journal of Pediatric Gastroenterology and Nutrition-JPGN |
ISSN: | 1536-4801 0277-2116 |
Popis: | Objectives: During pregnancy, small quantities of maternal cells are naturally transmitted to the fetus. This transmission, termed maternal microchimerism (MMc), has been implicated in autoimmune diseases but its potential role is unclear. We aimed to investigate if MMc at birth predicted childhood celiac disease (CD) risk, a common immune-mediated enteropathy often presenting in childhood. Methods: We designed a case-control study, nested in the Norwegian Mother, Father and Child Cohort. Participants were HLA class II typed to determine noninherited, nonshared maternal alleles (NIMA). Droplet digital (dd) PCR assays specific for common HLA class II NIMAs (HLA-DQB1∗03:01, ∗04:02 and ∗06:02/03) were used to estimate the quantity of maternal DNA, as a marker of maternal cells, in cord blood DNA from 124 children who later developed clinically diagnosed CD (median age at end of study 7.4 years, range 3.6–12.9) and 124 random controls. We tested whether presence of MMc was associated with CD using logistic regression, and compared ranks between cases and controls. Results: MMc, for example, maternal HLA antigens not inherited by the child, was found in 42% of cases and 43% of controls, and not associated with CD (odds ratio [OR] 0.97, 95% confidence interval [CI] 0.58–1.60). The ranks of MMc quantities in cases and controls were also similar (Mann-Whitney U-test, P = 0.71). The subgroup with HLA-DQB1:03∗01 as their NIMA had a potential association with MMc, where levels greater than median was associated with CD (OR 3.78, 95% CI 1.28–11.18). Conclusion: MMc measured in cord blood was not associated with later risk of CD. |
Databáze: | OpenAIRE |
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