Organoselenotriazoles attenuate oxidative damage induced by mitochondrial dysfunction in mev-1 Caenorhabditis elegans mutants
| Autor: | Simone Pinton, Diego Alves, Manoela do Sacramento, Willian Goulart Salgueiro, Jeferson Luis Franco, Luiz Brasil Lopes Rodrigues Junior, Daiana Silva Ávila, Cristiane de Freitas Rodrigues, Ana Helena de Castro Dal Forno, Riva de Paula Oliveira, Ana Thalita Gonçalves Soares |
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| Rok vydání: | 2018 |
| Předmět: |
Azides
Antioxidant medicine.medical_treatment 010501 environmental sciences Mitochondrion medicine.disease_cause 01 natural sciences Biochemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Paraquat medicine Animals Caenorhabditis elegans Caenorhabditis elegans Proteins Selenium Compounds 0105 earth and related environmental sciences chemistry.chemical_classification Reactive oxygen species biology Molecular Structure Cytochromes b biology.organism_classification Mitochondria Oxidative Stress chemistry Catalase Toxicity Mutation biology.protein Molecular Medicine Reactive Oxygen Species 030217 neurology & neurosurgery Oxidative stress |
| Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 53 |
| ISSN: | 1878-3252 |
| Popis: | Organic selenium compounds have several pharmacological activities already described, as anti-inflammatory and antitumor activities, which have been attributed to their antioxidant effects. Because they are promising in pharmacology, the synthesis of these compounds has increased significantly. As many new molecules are synthesized the use of a simple model like Caenorhabditis elegans is highly advantageous for initial evaluation of the toxicity and therapeutic potential of these molecules. The objective of this study was to evaluate the toxicity and antioxidant capacity of a series of selenotriazoles compounds in C. elegans. The animals were exposed to the compounds in liquid medium for only 30 min at the first larval stage (L1). The compounds had no toxic effects at the concentrations tested. Treatment with selenotriazoles (10 μM) partially reversed the stress induced by the pesticide paraquat (1 mM). Se-Tz Ia compound partially increased the survival of worms treated with H2O2 (0.5 mM). The compounds also increased the longevity of mev-1 mutants, which have a reduced life span by the production of excessive reactive oxygen species (ROS) in the mitochondria caused by a mutation in complex II of the electron transport chain. In addition, the compounds reduced the levels of ROS determined by the fluorescent probe DCF-DA as well as also reduced catalase enzyme activity in these animals. Based on the results found, it is possible to conclude that the compounds have antioxidant activity mainly in oxidative stress condition generated by a mitochondrial dysfunction in C. elegans. |
| Databáze: | OpenAIRE |
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