Comparison of the in vivo and in vitro genotoxicity of glyphosate isopropylamine salt in three different organisms
| Autor: | David Gómez Gallardo, Alfredo Feria Velasco, Mónica Reynoso Silva, Carlos Valdez Ramírez, Rafael León Sánchez, Carlos Alvarez-Moya, Alejandro Canales Aguirre |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
lcsh:QH426-470
DNA damage Tradescantia medicine.disease_cause Toxicology chemistry.chemical_compound glyphosate comet assay In vivo Genetics medicine Isopropylamine Molecular Biology biology genotoxicity biology.organism_classification Molecular biology In vitro Comet assay lcsh:Genetics chemistry Mutagenesis Glyphosate Genotoxicity Research Article |
| Zdroj: | Genetics and Molecular Biology, Vol 37, Iss 1, Pp 105-110 (2014) Genetics and Molecular Biology v.37 n.1 2014 Genetics and Molecular Biology Sociedade Brasileira de Genética (SBG) instacron:SBG Genetics and Molecular Biology, Volume: 37, Issue: 1, Pages: 105-110, Published: 2014 |
| ISSN: | 1415-4757 |
| Popis: | There is considerable controversy with regard to the genotoxicity of glyphosate, with some reports stating that this compound is non-toxic for fish, birds and mammals. In this work, we used the comet assay to examine the genotoxicity of glyphosate isopropylamine (0.7, 7, 70 and 700 µM) in human lymphocytes, erythrocytes of Oreochromis niloticus and staminal nuclei of Tradescantia (4430) in vitro and in vivo. Cells, nuclei and fish that had and had not been exposed to 5 mM N-nitrosodiethylamine (NDEA) were used as positive and negative controls, respectively. Significant (p < 0.01) genetic damage was observed in vivo and in vitro in all cell types and organisms tested. Human lymphocytes and Tradescantia hairs showed lower genetic damage in vivo compared to in vitro, possibly because of efficient metabolization of the herbicide. In O. niloticus erythrocytes, significant (p < 0.001) genotoxicity was observed at > 7 µM, whereas in vitro, glyphosphate was genotoxic in human lymphocytes and Tradescantia hairs at > 0.7 µM. These results indicate that glyphosate is genotoxic in the cells and organisms studied at concentrations of 0.7-7 µM. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|