Correlations between in vitro potency of polyethylene glycol–protein conjugates and their chromatographic behavior

Autor: Menci Kunstelj, Simon Caserman, Simona Jevsevar, Tanja Milunović, Mateja Kusterle, Vladka Gaberc Porekar, Mark Schiefermeier
Rok vydání: 2009
Předmět:
Zdroj: Analytical Biochemistry. 389:27-31
ISSN: 0003-2697
Popis: Pegylation is the most widely used and accepted methodology for half-life extension of biopharmaceutical drugs that also improves physicochemical and biological characteristics of proteins considerably. Most of the positive pharmacological effects of pegylated proteins are believed to be related to an increased hydrodynamic volume and molecular size. To explore the size impact of polyethylene glycol (PEG) on in vitro potency, a series of well-defined conjugates of interferon alpha-2b (IFN) were prepared with PEGs of different lengths and shapes specifically attached to the N-terminal amino group of the protein. Specificity of the attachment was confirmed by peptide mapping and mass spectroscopy. When potency values determined by reporter gene assay were correlated with methods for molecular weight and size characterization, such as size exclusion chromatography and dynamic light scattering, rough parallels were found. Unexpectedly, the retention times on cation exchange chromatography showed much higher correlation with experimentally determined in vitro potency. It appears that in a series of N-terminally pegylated IFNs, their in vitro potency could be predicted from the retention times on the cation exchange chromatography columns, probably because both methods reflect not only the influence of molecular size but also the impact of protein masking exerted by attached PEG moiety.
Databáze: OpenAIRE
Externí odkaz: