Biochemical diversity within the 'Mycoplasma mycoides cluster'
| Autor: | Roger J. Miles, H. Varsani, R. R. Taylor, R. H. Leach, E. A. M. Abu-Groun, B. J. Wadher |
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| Rok vydání: | 1994 |
| Předmět: |
Glycerol
Cattle Diseases Sheep Diseases Mannose Microbiology Mycoplasma capricolum chemistry.chemical_compound Contagious caprine pleuropneumonia Mycoplasma Species Specificity Pneumonia Mycoplasma Pyruvic Acid medicine Animals Lactic Acid Pyruvates Goat Diseases Sheep biology Goats biology.organism_classification medicine.disease Trehalose Oxygen chemistry Biochemistry Chemotaxonomy Lactates Mollicutes Carbohydrate Metabolism Cattle Mycoplasma mycoides Bacteria |
| Zdroj: | Microbiology. 140:2033-2042 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/13500872-140-8-2033 |
| Popis: | The metabolism of 51 strains within the ‘Mycoplasma mycoides cluster’ was investigated by measuring oxygen uptake following the addition of organic substrates to washed cell suspensions. There were extensive differences between strains in the range of substrates utilized, the relative rates of oxidation and the observed saturation constants for substrates, which ranged from a few μM to several mM. M. mycoides subsp. capri and M. mycoides subsp. mycoides LC (large colony) strains were diverse and could not be distinguished by substrate utilization patterns. However, there were consistent differences in the patterns of substrate utilization between other groups of the M. mycoides cluster, suggesting that these patterns may be useful in identification. In particular, SC (small colony) strains of M. mycoides subsp. mycoides were distinguished by their inability to oxidize maltose, trehalose and (at low concentrations) mannose and glucosamine. Surprisingly, the type strain, M. F38, of Mycoplasma capricolum subsp. capripneumoniae and two further isolates differed from all other strains in that they did not oxidize glucose or other sugars. They did, however, oxidize pyruvate, lactate and 2-oxobutyrate at high rates. The marked metabolic differences between these strains and M. capricolum subsp. capricolum strains is in contrast to the genetic evidence that was used to support the designation of the M. F38 group as a subspecies of M. capricolum. |
| Databáze: | OpenAIRE |
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