Tlr4 participates in the responses of markers of apoptosis, inflammation, and ER stress to different acute exercise intensities in mice hearts
Autor: | Alisson L. da Rocha, Dennys E. Cintra, Fabiani Gai Frantz, Rafael Calais Gaspar, Ana P. Pinto, Lilian Eslaine Costa Mendes da Silva, Eduardo R. Ropelle, Fernando Moreira Simabuco, Adelino Sanchez Ramos da Silva, Larissa G. de Vicente, Vitor Rosetto Muñoz, Rafael L. Rovina, José Rodrigo Pauli, Leandro Pereira de Moura |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
medicine.medical_specialty Physical Exertion Apoptosis Physical exercise Inflammation 030226 pharmacology & pharmacy General Biochemistry Genetics and Molecular Biology Mice 03 medical and health sciences 0302 clinical medicine Internal medicine Animals Medicine General Pharmacology Toxicology and Pharmaceutics Receptor Creatine Kinase Mice Knockout Tumor Necrosis Factor-alpha business.industry Endoplasmic reticulum Interleukin-17 Computational Biology Heart General Medicine PROTEÍNAS Endoplasmic Reticulum Stress Mice Inbred C57BL Toll-Like Receptor 4 030104 developmental biology Endocrinology Echocardiography Unfolded protein response TLR4 Signal transduction medicine.symptom business Signal Transduction |
Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
ISSN: | 0024-3205 |
Popis: | Background Toll-like receptor 4 (Tlr4) is recognized due to its role in the immune response. Also, this protein can participate in the signaling pathway of events triggered by physical exercise such as apoptosis, inflammation, and endoplasmic reticulum (ER) stress. The main objective of this study was to evaluate the role of Tlr4 in the markers of these events in the myocardium of mice submitted to acute physical exercise (APE) protocols at different intensities. Methods Echocardiogram, RT-qPCR, and immunoblotting technique were used to evaluate the left ventricle of wild-type (WT) and Tlr4 knockout (Tlr4 KO) submitted to APE protocols at 45, 60, and 75% of their maximal velocity. Also, we performed the bioinformatics analysis to establish the connection of heart mRNA levels of Tlr4 with heart genes of inflammation and ER stress of several isogenic strains of BXD mice. Results Under basal conditions, the Tlr4 deletion diminished the performance, and expression of inflammation and ER stress genes in the left ventricle, but increased the serum levels of CK, Il-17, and Tnf-alpha. Under the same exercise conditions, the Tlr4 deletion reduced the glycemia, serum levels of CK, Il-17, and Tnf-alpha, as well as genes and/or proteins related to apoptosis, inflammation and ER stress in the left ventricle, but increased the levels of CK-mb and LDH, as well as other genes related to apoptosis, inflammation, and ER stress in the left ventricle. Conclusion Altogether, the current findings highlighted the effects of different acute exercise intensities were attenuated in the heart of Tlr4 KO mice. |
Databáze: | OpenAIRE |
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