Reduction of Nanoparticle Load in Cells by Mitosis but Not Exocytosis
| Autor: | Miguel Spuch-Calvar, Barbara Rothen-Rutishauser, Dimitri Vanhecke, Alke Petri-Fink, Lukas Steinmetz, Sandor Balog, Patricia Taladriz-Blanco, Joël Bourquin, Dedy Septiadi |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Cell division
Surface Properties Cell Mitosis General Physics and Astronomy 02 engineering and technology 010402 general chemistry 01 natural sciences Exocytosis Mice Live cell imaging medicine Animals General Materials Science Particle Size Cells Cultured Chemistry Vesicle Optical Imaging General Engineering Mononuclear phagocyte system Silicon Dioxide 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure Biophysics Nanoparticles Gold Lysosomes 0210 nano-technology Oxidation-Reduction Porosity Intracellular |
| Zdroj: | ACS Nano. 13:7759-7770 |
| ISSN: | 1936-086X 1936-0851 |
| Popis: | The long-term fate of biomedically relevant nanoparticles (NPs) at the single cell level after uptake is not fully understood yet. We report that lysosomal exocytosis of NPs is not a mechanism to reduce the particle load. Biopersistent NPs such as nonporous silica and gold remain in cells for a prolonged time. The only reduction of the intracellular NP number is observed via cell division, e.g., mitosis. Additionally, NP distribution after cell division is observed to be asymmetrical, likely due to the inhomogeneous location and distribution of the NP-loaded intracellular vesicles in the mother cells. These findings are important for biomedical and hazard studies as the NP load per cell can vary significantly. Furthermore, we highlight the possibility of biopersistent NP accumulation over time within the mononuclear phagocyte system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|