Isolation and functional characterization of SUCROSE SYNTHASE 1 and SUCROSE TRANSPORTER 2 promoters from ramie (Boehmeria nivea L. Gaudich)
| Autor: | Pingan Guo, Yancheng Zheng, Lijun Liu, Yanyan He, Bo Wang, Dingxiang Peng |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Sucrose Arabidopsis Boehmeria Ramie 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Gene Expression Regulation Plant Genetics Cloning Molecular Promoter Regions Genetic Gene Phylogeny Plant Proteins biology Membrane Transport Proteins Transporter Promoter Exons General Medicine Plants Genetically Modified biology.organism_classification Introns Phenotype 030104 developmental biology chemistry Biochemistry Glucosyltransferases 030220 oncology & carcinogenesis biology.protein Sucrose synthase |
| Zdroj: | Gene. 685:114-124 |
| ISSN: | 0378-1119 |
| Popis: | Sucrose synthase and sucrose transporter are involved in sucrose metabolism and partitioning of photosynthetic products, respectively. In this study, we cloned SUCROSE SYNTHASE 1 and SUCROSE TRANSPORTER 2 genes from ramie. Real-time quantitative PCR revealed that BnSUS1 and BnSUT2 were widely expressed in the analyzed tissues. Subsequently, the two promoters of BnSUS1 and BnSUT2 were isolated and truncated. The two promoters and their truncated fragments were fused GUS to transform into Arabidopsis. GUS staining showed that BnSUS1pro-1690 and BnSUS1pro-1420 had vascular specificity in cotyledons and mature leaves while BnSUT2pro-2239, BnSUT2pro-1681, BnSUT2pro-1199 and BnSUT2pro-618 had a constitutive function in seedlings and mature organs. Notably, the activity of BnSUT2pro-2239 and its fragments (except that of BnSUT2pro-231) are strongly induced by mechanical wounding. Moreover, BnSUS1pro-1051 and BnSUS1pro-485 are sensitive to CuSO4 treatment while BnSUT2pro-2239 and BnSUT2pro-1681 are sensitive to PEG and ABA treatments, respectively. Our findings will provide the foundation for deciphering the functions of BnSUS1 and BnSUT2, and also expand the promoter library to provide more options for plant genetic engineering. |
| Databáze: | OpenAIRE |
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