Functional expression of murine LRP1 requires correction of Lrp1 cDNA sequences
| Autor: | Annick Lauwers, Stefan M. Willems, Anton J.M. Roebroek, Els Thiry, Liesbet Smeijers, Fred Van Leuven |
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| Rok vydání: | 2002 |
| Předmět: |
DNA
Complementary Sequence analysis Mutant Biophysics Mutagenesis (molecular biology technique) CHO Cells Biology medicine.disease_cause Biochemistry Mice Exon Structural Biology Cricetinae Complementary DNA Genetics medicine Animals Amino Acid Sequence Peptide sequence Mutation Chinese hamster ovary cell Sequence Analysis DNA Molecular biology Mice Inbred CBA Low Density Lipoprotein Receptor-Related Protein-1 |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1577:155-158 |
| ISSN: | 0167-4781 |
| DOI: | 10.1016/s0167-4781(02)00419-0 |
| Popis: | Here, we describe the reconstruction of a functional 14 kbp full-length murine Lrp1 cDNA from overlapping partial cDNAs, which were described before [Biochim. Biophys. Acta 1173 (1993) 71]. The reconstructed full-length cDNA needed sequence correction (by mutagenesis) due to nucleotide errors present in the underlying partial cDNAs. These mistakes compromised the proteolytical maturation of the LRP precursor (4545 aa) into its alpha- and beta-subunits. To identify these mistakes initially, detailed sequence analyses and comparison of genomic and cDNA sequences of different murine strains proved to be necessary to obtain correct wild-type sequences. Comparison of Lrp1 cDNA sequences of CBA mice with Lrp1 genomic exon sequences of 129P3/J mice (like in man 89 exons) revealed only 24 nucleotide differences within about 14.8 kbp. Only 1 out of 23 nucleotide differences in the protein coding region affected an amino acid residue: Thr versus Ala at amino acid residue position 2642 in 129P3/J and CBA, respectively. After correction by mutagenesis, both a 129P3/J and a CBA-based version of a full-length wild-type Lrp1 cDNA were functionally expressed in an LRP-deficient mutant CHO cell line. Transient expression showed the expected maturation of the LRP precursor into its two subunits. Furthermore, stable transfection restored the sensitivity to exposure to Pseudomonas aeruginosa toxin A (PEA). Since LRP is the unique receptor for this toxin, this indicates that the toxin could enter the cells after binding to and endocytosis by its genuine receptor. This murine LRP expression system will be instrumental in future experimental dissection of this multifunctional receptor. |
| Databáze: | OpenAIRE |
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