Comparison of periprosthetic tissue digestion methods for ultra-high molecular weight polyethylene wear debris extraction
| Autor: | Ryan M. Baxter, Joanne L. Tipper, Marla J. Steinbeck, Michele Marcolongo, S. M. Kurtz, Javad Parvizi |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Caustics Potassium Compounds Swine Biomedical Engineering Nitric Acid Article Biomaterials chemistry.chemical_compound Digestion (alchemy) Materials Testing Hydroxides Animals Humans Sodium Hydroxide Composite material Ultra-high-molecular-weight polyethylene Chromatography biology Extraction (chemistry) Acetabulum Polyethylene Proteinase K Prosthesis Failure chemistry Sodium hydroxide Reagent biology.protein Particle Indicators and Reagents Hip Prosthesis Polyethylenes |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. :409-418 |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.31416 |
| Popis: | There is considerable interest in characterization of wear debris from polyethylene (UHMWPE) bearing components used in total joint replacement. To isolate UHMWPE wear debris, tissue samples must be excised from regions adjacent to revised UHMWPE implant components, followed by exposure to one of many available tissue digestion methods. Numerous studies demonstrate successful digestion, but the relative efficiency of each method is not clear. The purpose of this study was to evaluate a variety of conditions for tissue digestion to provide a quantitative comparison of methods. Porcine and human hip tissues were exposed for 24 h to basic, acidic or enzymatic agents, filtered and digestion efficiency calculated based on the percentage of initial to final tissue weight. Of the conditions tested, 5 M NaOH, 5 M KOH, 15 M KOH or 15.8 M HNO3 yielded the most complete porcine hip tissue digestion ( |
| Databáze: | OpenAIRE |
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