The murine ortholog of matrix metalloproteinase 19: its cloning, gene organization, and expression
| Autor: | Justine Kusch, Cornelia Kolb, Thorsten Sadowski, Mona Harnasch, Radislav Sedlacek, Markus Mueller |
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| Rok vydání: | 2000 |
| Předmět: |
DNA
Complementary TATA box Molecular Sequence Data Gene Expression Biology Regulatory Sequences Nucleic Acid DNA sequencing Gene Expression Regulation Enzymologic Exon Mice Complementary DNA Matrix Metalloproteinases Secreted Genetics Tumor Cells Cultured Animals Humans Tissue Distribution Amino Acid Sequence RNA Messenger Cloning Molecular Gene Cloning Mice Inbred BALB C Base Sequence Sequence Homology Amino Acid Intron Metalloendopeptidases General Medicine 3T3 Cells DNA Exons Sequence Analysis DNA Molecular biology Immunohistochemistry Introns genomic DNA Ecdysterone Genes Sequence Alignment |
| Zdroj: | Gene. 256(1-2) |
| ISSN: | 0378-1119 |
| Popis: | We have isolated a murine cDNA orthologous to the human matrix metalloproteinase 19 (hMMP-19). The murine MMP-19 cDNA was amplified by RT–PCR using specific primers whose DNA sequences were derived from both murine MMP-19 genomic DNA and partial cDNA sequences. The murine MMP-19 (mMMP-19) is 79% identical to the human ortholog and encodes a protein of 527 amino acids with a deduced molecular mass of 59.1 kDa. Analyzing the exon/intron junctions we revealed that the murine MMP-19 gene consists of nine exons and eight introns, and thus differs from the gene organization of other matrix metalloproteinases. Furthermore, a 587 bp fragment of the mMMP-19 promoter containing a TATA box and an AP-1 binding motif was cloned, and 3.3 kb transcripts of the MMP-19 gene were identified in liver, kidney, spleen, and colon. Finally, immunostaining of murine heart cryosections showed that mMMP-19, like its human counterpart, is expressed in the arterial tunica media of large blood vessels. By cloning mMMP-19 and unraveling its genomic structure, we have obtained valuable information for further study of the function of this MMP in vivo. |
| Databáze: | OpenAIRE |
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