Identification and Characterization of microRNAS from Entamoeba histolytica HM1-IMSS
| Autor: | Jaime Fco. Treviño-Neávez, Victor Trevino, Diana Reséndez-Pérez, M. Morales-Vallarta, Jannet E. Salinas-Hernández, Jorge A. Verduzco-Martínez, M.P. Barrón-González, Fermín Mar-Aguilar, Marcela M. Taméz-Guerrero, Eufemia Morales-Rubio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Small RNA
sequence analysis microRNA 46 Microarrays microRNA 47 Genes Protozoan Gene Identification and Analysis lcsh:Medicine Protozoology Genome Biochemistry Nucleic Acids Molecular Cell Biology Databases Genetic Cluster Analysis microRNA 144 Genome Sequencing lcsh:Science genome analysis Oligonucleotide Array Sequence Analysis microRNA 5 Multidisciplinary microRNA 2 biology microRNA article bioinformatics Genomics RNA analysis unclassified drug protozoal RNA real time polymerase chain reaction Research Article microRNA 29 Sequence analysis microRNA 24 biochip DNA sequence Computational biology Real-Time Polymerase Chain Reaction Microbiology Deep sequencing DNA sequencing Molecular Genetics Entamoeba Histolytica Entamoeba histolytica parasitic diseases Genetics controlled study Trophozoites gene library microRNA 8 Gene Biology nonhuman microRNA 13 Sequence Analysis RNA lcsh:R Computational Biology Reproducibility of Results sequence homology microRNA 12 biology.organism_classification Molecular biology trophozoite MicroRNAs 7 INGENIERÍA Y TECNOLOGÍA gene expression RNA Parastic Protozoans lcsh:Q microarray analysis microfluidic analysis |
| Zdroj: | PLoS ONE PLoS ONE, Vol 8, Iss 7, p e68202 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Background:Entamoeba histolytica is the causative agent of amebiasis, a disease that is a major source of morbidity and mortality in the developing world. MicroRNAs (miRNAs) are a large group of non-coding RNAs that play important roles in regulating gene expression and protein translation in animals. Genome-wide identification of miRNAs is a critical step to facilitating our understanding of genome organization, genome biology, evolution, and post-transcriptional regulation.Methodology/Principal Findings:We sequenced a small RNA library prepared from a culture of trophozoites of Entamoeba histolytica Strain HM1-IMSS using a deep DNA sequencing approach. Deep sequencing yielded 16 million high-quality short sequence reads containing a total of 5 million non-redundant sequence reads. Based on a bioinformatics pipeline, we found that only 0.5% of these non-redundant small RNA reads were a perfect match with the drafted E. histolytica genome. We did not find miRNA homologs in plant or animal miRNAs. We discovered 199 new potential Entamoeba histolytica miRNAs. The expression and sequence of these Ehi-miRNAs were further validated through microarray by μParaflo Microfluidic Biochip Technology. Ten potential miRNAs were additionally confirmed by real time RT-PCR analysis. Prediction of target genes matched 32 known genes and 34 hypothetical genes.Conclusions/Significance:These results show that there is a number of regulatory miRNAs in Entamoeba histolytica. The collection of miRNAs in this parasite could be used as a new platform to study genomic structure, gene regulation and networks, development, and host-parasite interactions. © 2013 Mar-Aguilar et al. |
| Databáze: | OpenAIRE |
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