Improved double immunoenzyme labelling using alkaline phosphatase and horseradish peroxidase

Autor: NJ Malik, ME Daymon
Rok vydání: 1982
Předmět:
Zdroj: Journal of Clinical Pathology. 35:1092-1094
ISSN: 0021-9746
DOI: 10.1136/jcp.35.10.1092
Popis: Alkaline phosphatase and peroxidase complexes were applied to tissue sections simultaneously to label two antigens. An azo-coupling method was used to demonstrate the alkaline phosphatase activity. The final result gave clear contrast between the two antigens and allowed the use of haematoxylin as the nuclear counterstain.
Databáze: OpenAIRE