Improved double immunoenzyme labelling using alkaline phosphatase and horseradish peroxidase
Autor: | NJ Malik, ME Daymon |
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Rok vydání: | 1982 |
Předmět: |
biology
Review Article General Medicine Counterstain Haematoxylin Alkaline Phosphatase Horseradish peroxidase Pathology and Forensic Medicine Immunoenzyme Techniques chemistry.chemical_compound Tissue sections chemistry Biochemistry Antigen Labelling parasitic diseases Methods biology.protein Alkaline phosphatase Hematoxylin Horseradish Peroxidase Peroxidase |
Zdroj: | Journal of Clinical Pathology. 35:1092-1094 |
ISSN: | 0021-9746 |
DOI: | 10.1136/jcp.35.10.1092 |
Popis: | Alkaline phosphatase and peroxidase complexes were applied to tissue sections simultaneously to label two antigens. An azo-coupling method was used to demonstrate the alkaline phosphatase activity. The final result gave clear contrast between the two antigens and allowed the use of haematoxylin as the nuclear counterstain. |
Databáze: | OpenAIRE |
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