Interactions of Mycoplasma bovoculi with Erythrocytes: Role of p94 Surface Protein
Autor: | B. A. Salih, Ricardo F. Rosenbusch |
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Rok vydání: | 1999 |
Předmět: |
Erythrocytes
medicine.drug_class Blotting Western Cattle Diseases Neuraminidase Sulfur Radioisotopes Monoclonal antibody medicine.disease_cause Mycoplasma bovoculi Mice Mycoplasma Antigen medicine Animals Hemadsorption Mycoplasma Infections Trypsin Receptor Mice Inbred BALB C biology Hemagglutination Antibodies Monoclonal General Medicine Molecular biology biology.protein Scintillation Counting Cattle Surface protein Bacterial Outer Membrane Proteins medicine.drug |
Zdroj: | Journal of Veterinary Medicine, Series B. 46:323-329 |
ISSN: | 0931-1793 |
Popis: | Summary The attachment of two strains of Mycoplasma bovoculi to erythrocytes was measured using 35S-methionine-labelled organisms. Receptor sites of M. bovoculi involved in this attachment are trypsin-sensitive, since mild trypsin treatment of the intact organisms abolished this process completely. Pretreatment of erythrocyt***es with trypsin or increasing concentrations of neuraminidase resulted in no measurable effect. Monoclonal antibody MA25.5 directed against a M. bovoculi surface antigen of 94 kDa termed p94 blocked 40% of the attachment, while MA18.13 directed against a 57 kDa protein band of M. bovoculi had no effect on the attachment process. Other properties of M. bovoculi were tested using six strains of the mycoplasma and erythrocytes from several animal species. None of the strains showed haemagglutinating or haemadsorbing activities. |
Databáze: | OpenAIRE |
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