Temperature Regulation of the Hemin Storage (Hms + ) Phenotype of Yersinia pestis Is Posttranscriptional
| Autor: | Alexander G. Bobrov, Olga Kirillina, Lisa L. Pedersen, Robert D. Perry, Jacqueline D. Fetherston, Jennifer Abney, Heather A. Jones |
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| Rok vydání: | 2004 |
| Předmět: |
Transcription
Genetic Yersinia pestis Operon Biology Microbiology chemistry.chemical_compound Bacterial Proteins Transcription (biology) Inner membrane Gene Regulation Molecular Biology Regulation of gene expression Temperature Membrane Proteins Promoter Gene Expression Regulation Bacterial Molecular biology Culture Media Phenotype Membrane protein chemistry Mutation Hemin Bacterial outer membrane Plasmids |
| Zdroj: | Journal of Bacteriology. 186:1638-1647 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | In Yersinia pestis , the Congo red (and hemin) binding that is characteristic of the Hms + phenotype occurs at temperatures up to 34°C but not at higher temperatures. Manifestation of the Hms + phenotype requires at least five proteins (HmsH, -F, -R, -S, and -T) that are organized into two separate operons: hmsHFRS and hmsT. HmsH and HmsF are outer membrane proteins, while HmsR, HmsS, and HmsT are predicted to be inner membrane proteins. We have used transcriptional reporter constructs, RNA dot blots, and Western blots to examine the expression of hms operons and proteins. Our studies indicate that transcription from the hmsHFRS and hmsT promoters is not regulated by the iron status of the cells, growth temperature, or any of the Hms proteins. In addition, the level of mRNA for both operons is not significantly affected by growth temperature. However, protein levels of HmsH, HmsR, and HmsT in cells grown at 37°C are very low compared to those in cells grown at 26°C, while the amounts of HmsF and HmsS show only a moderate reduction at the higher growth temperature. Neither the Pla protease nor a putative endopeptidase (Y2360) encoded upstream of hmsH is essential for temperature regulation of the Hms + phenotype. However, HmsT at 37°C is sensitive to degradation by Lon and/or ClpPX. Thus, the stability of HmsH, HmsR, and HmsT proteins likely plays a role in temperature regulation of the Hms + phenotype of Y. pestis . |
| Databáze: | OpenAIRE |
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