Epigenetic mapping of the somatotropic axis in Nile tilapia reveals differential DNA hydroxymethylation marks associated with growth
| Autor: | Pål Sætrom, Dafni Anastasiadi, Robin Mjelle, Francesc Piferrer, Jorge M.O. Fernandes, Artem V. Nedoluzhko, Tomasz Krzysztof Podgorniak, Ioannis Konstantinidis |
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| Přispěvatelé: | European Research Council, European Commission, Research Council of Norway, Agencia Estatal de Investigación (España) |
| Rok vydání: | 2021 |
| Předmět: |
DNA Hydroxymethylation
Somatotropic cell Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Zoofysiologi og komparativ fysiologi: 483 [VDP] Growth Biology Article Teleosts Epigenesis Genetic Cytosine 03 medical and health sciences DNA hydroxymethylation 0302 clinical medicine Gene expression Genetics Animals Epigenetics Gene 030304 developmental biology Regulation of gene expression 0303 health sciences Somatotropic axis Myogenesis Promoter Cichlids DNA DNA Methylation Cell biology Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474 [VDP] 5-Methylcytosine 030217 neurology & neurosurgery |
| Zdroj: | Genomics Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0888-7543 |
| DOI: | 10.1016/j.ygeno.2021.06.037 |
| Popis: | 12 pages, 6 figures, 2 tables, supplementary material https://doi.org/10.1016/j.ygeno.2021.06.037.-- Data availability: The DNA hydroxymethylation dataset of this study is available in the SRA (NCBI) repository, under the accession number PRJNA665628. The RNA sequencing dataset of this study is available in the GEO (NCBI) repository, under the accession number GSE158910 In vertebrates, the somatotropic axis comprising the pituitary gland, liver and muscle plays a major role in myogenesis. Its output in terms of muscle growth is highly affected by nutritional and environmental cues, and thus likely epigenetically regulated. Hydroxymethylation is emerging as a DNA modification that modulates gene expression but a holistic characterization of the hydroxymethylome of the somatotropic axis has not been investigated to date. Using reduced representation 5-hydroxymethylcytosine profiling we demonstrate tissue-specific localization of 5-hydroxymethylcytosines at single nucleotide resolution. Their abundance within gene bodies and promoters of several growth-related genes supports their pertinent role in gene regulation. We propose that cytosine hydroxymethylation may contribute to the phenotypic plasticity of growth through epigenetic regulation of the somatotropic axis This work was funded by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation programme (grant agreements nos. 683210 and 812986) and by the Research Council of Norway under the Toppforsk programme (grant agreement no 250548/F20) With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S) |
| Databáze: | OpenAIRE |
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