Post-transcriptional markers associated with clinical complications in Type 1 and Type 2 diabetes mellitus
| Autor: | Cinthia C. Alves, Matheus Costa e Silva, Sergio Atala Dib, Diane M. Rassi, Juliana Doblas Massaro, Maria Cristina Foss-Freitas, Daniel Guariz Pinheiro, Elza Tiemi Sakamoto-Hojo, Eduardo Antônio Donadi, Geraldo Aleixo Silva Passos, Claudia Danella Polli, Felipe Crispim, Wallace Rodrigues de Holanda Miranda, Nathalia Joanne Bispo Cezar |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Adolescent Transcription Genetic MICRORNAS 030209 endocrinology & metabolism Type 2 diabetes Bioinformatics Biochemistry Nephropathy 03 medical and health sciences Young Adult 0302 clinical medicine Endocrinology Diabetes mellitus microRNA medicine Cluster Analysis Data Mining Humans Gene Regulatory Networks Molecular Biology Aged Diabetes Complication Type 1 diabetes Massive parallel sequencing business.industry Gene Expression Profiling Type 2 Diabetes Mellitus Reproducibility of Results Middle Aged medicine.disease MicroRNAs 030104 developmental biology Diabetes Mellitus Type 1 Diabetes Mellitus Type 2 Female business Biomarkers |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1872-8057 |
| Popis: | The delayed diagnosis and the inadequate treatment of diabetes increase the risk of chronic complications. The study of regulatory molecules such as miRNAs can provide expression profiles of diabetes and diabetes complications. We evaluated the mononuclear cell miRNA profiles of 63 Type 1 and Type 2 diabetes patients presenting or not microvascular complications, and 40 healthy controls, using massive parallel sequencing. Gene targets, enriched pathways, dendograms and miRNA-mRNA networks were performed for the differentially expressed miRNAs. Six more relevant miRNAs were validated by RT-qPCR and data mining analysis. MiRNAs associated with specific complications included: i) neuropathy (miR-873-5p, miR-125a-5p, miR-145-3p and miR-99b-5p); ii) nephropathy (miR-1249-3p, miR-193a-5p, miR-409-5p, miR-1271-5p, miR-501-3p, miR-148b-3p and miR-9-5p); and iii) retinopathy (miR-143-3p, miR-1271-5p, miR-409-5p and miR-199a-5p). These miRNAs mainly targeted gene families and specific genes associated with advanced glycation end products and their receptors. Sets of miRNAs were also defined as potential targets for diabetes/diabetes complication pathogenesis. |
| Databáze: | OpenAIRE |
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