Protection of neuronal cells against reactive oxygen species by carnosine and related compounds
| Autor: | Shiori Tsubone, Irina Petrushanko, Alexander Boldyrev, Toomas A. Leinsoo, E. R. Bulygina, Hiroki Abe |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Antioxidant
N-Methylaspartate Physiology medicine.medical_treatment Anserine Carnosine Biochemistry Flow cytometry chemistry.chemical_compound Cerebellum medicine Animals Rats Wistar Molecular Biology Histidine chemistry.chemical_classification Neurons Reactive oxygen species medicine.diagnostic_test Flow Cytometry Rats Spectrometry Fluorescence chemistry NMDA receptor Reactive Oxygen Species Intracellular |
| Zdroj: | Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 137(1) |
| ISSN: | 1096-4959 |
| Popis: | Carnosine and related compounds were compared in terms of their abilities to decrease the levels of reactive oxygen species (ROS) in suspensions of isolated neurons activated by N-methyl-D-aspartic acid (NMDA) using both stationary fluorescence measurements and flow cytometry. Carnosine was found to suppress the fluorescent signal induced by ROS production and decreased the proportion of highly fluorescent neurons, while histidine showed opposite effects. N-Acetylated derivatives of both carnosine and histidine demonstrated weak (statistically indistinguishable) suppressive effects on the ROS signal. N-Methylated derivatives of carnosine suppressed intracellular ROS generation to the same extent as carnosine. This rank of effectiveness is distinct from that previously obtained for the anti-radical ability of CRCs (anserine>carnosine>ophidine). These differences suggest that the similar ability of carnosine and its N-methylated derivatives to protect neuronal cells against the excitotoxic effect of NMDA is not solely related to the antioxidant properties of these compounds. |
| Databáze: | OpenAIRE |
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