Hyperoxia reduces salivary secretion by inducing oxidative stress in mice
| Autor: | Takuya Miyawaki, Ayako Tajiri, Hitoshi Higuchi |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Saliva Submandibular Gland Hyposalivation Hyperoxia medicine.disease_cause Salivary Glands Superoxide dismutase 03 medical and health sciences Mice 0302 clinical medicine Superoxide Dismutase-1 stomatognathic system Internal medicine medicine Animals Secretion RNA Messenger General Dentistry TUNEL assay biology Chemistry Superoxide Dismutase Pilocarpine Membrane Proteins 030206 dentistry Cell Biology General Medicine respiratory system Submandibular gland Mice Inbred C57BL Oxygen Oxidative Stress 030104 developmental biology medicine.anatomical_structure Endocrinology Otorhinolaryngology Amylases biology.protein medicine.symptom Salivation Oxidative stress Heme Oxygenase-1 medicine.drug |
| Zdroj: | Archives of oral biology. 98 |
| ISSN: | 1879-1506 |
| Popis: | OBJECTIVE: The aim of this study was to determine the effects of prolonged hyperoxia on salivary glands and salivary secretion in mice. DESIGN: Male C57BL/6 J mice were kept in a 75% oxygen chamber (hyperoxia group) or a 21% oxygen chamber for 5 days. We measured the secretion volume, protein concentration, and amylase activity of saliva after the injection of pilocarpine. In addition, we evaluated the histological changes induced in the submandibular glands using hematoxylin and eosin and Alcian blue staining and assessed apoptotic changes using the TdT-mediated dUTP nick end labeling (TUNEL) assay. We also compared the submandibular gland expression levels of heme oxygenase-1 (HO-1), superoxide dismutase (SOD)-1, and SOD-2 using the real-time polymerase chain reaction. RESULTS: In the hyperoxia group, salivary secretion was significantly inhibited at 5 and 10 min after the injection of pilocarpine, and the total salivary secretion volume was significantly decreased. The salivary protein concentration and amylase activity were also significantly higher in the hyperoxia group. In the histological examinations, enlargement of the mucous acini and the accumulation of mucins were observed in the submandibular region in the hyperoxia group, and the number of TUNEL-positive cells was also significantly increased in the hyperoxia group. Moreover, the expression levels of HO-1, SOD-1, and SOD-2 were significantly higher in the hyperoxia group. CONCLUSION: Our results suggest that hyperoxia reduces salivary secretion, and oxidative stress reactions might be involved in this. |
| Databáze: | OpenAIRE |
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