Animal model of coronary microembolization under transthoracic echocardiographic guidance in rats
| Autor: | Manlu Fan, Weixin Sun, Huaqin Tong, Haowen Zhang, Jie Han, Sheng-Feng Lu, Tong Sun, Songyi Cheng, Xiaohu Chen, Xuan Han, Jiandong Chen, Qian Zhang |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Cardiac fibrosis medicine.medical_treatment Embolism education Biophysics Lung injury Biochemistry Fibrin Rats Sprague-Dawley Animal model Internal medicine medicine Animals Thoracotomy Molecular Biology Ejection fraction biology business.industry Postoperative complication Thrombosis Cell Biology medicine.disease Coronary Vessels Disease Models Animal Echocardiography Cardiology biology.protein Breathing business |
| Zdroj: | Biochemical and Biophysical Research Communications. 568:174-179 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2021.05.045 |
| Popis: | The aim of the study was to develop a model of coronary microembolization (CME) in rats at a lower cost. We developed a novel rat model without thoracotomy and ventilation under the guidance of echocardiography. Rats were sacrificed at 3 h, 24 h and 1 month postoperatively in both the Echo-CME and Open-chest CME groups for the comparison of the modeling accuracy, mortality, cardiopulmonary circulation, pleural adhesion and ventilation-induced lung injury (VILI). Results showed that the coronary microthrombus formed at 3 h and reached its peak at 24 h postoperatively, which included platelet aggregation and fibrin web. The Echo-group increases success rates, decreased mortality, postoperative complications including pleural adhesion, cardiopulmonary dysfunction and VILI postoperatively than the Open-chest group at 1month postoperatively. The ejection fraction of the CME group decreased to 50% and obvious cardiac fibrosis formed at 3 months postoperatively. Our unique surgical method provided a platform to study molecular mechanisms and potential new pathways for CME treatment. |
| Databáze: | OpenAIRE |
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