Interactions of Avocado (Persea americana) Cytochrome P-450 with Monoterpenoids

Autor:	Roger M. Wallsgrove, Jevon M. West, D. L. Hallahan, Jonathan H. A. Nugent, Glenn W. Dawson, D. W. M. Smiley, Beverly J. Hallahan
Rok vydání:	1992
Předmět:	Persea Cytochrome biology Physiology Stereochemistry Monoterpene food and beverages Cytochrome P450 Plant Science biology.organism_classification Hydroxylation chemistry.chemical_compound Biochemistry chemistry Genetics biology.protein Nerol Geraniol Metabolism and Enzymology Demethylation
Zdroj:	Plant Physiology. 98:1290-1297
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.98.4.1290
Popis:	The microsomal fraction of avocado (Persea americana) mesocarp is a rich source of cytochrome P-450 active in the demethylation of xenobiotics. Cytochrome P-450 from this tissue has been purified and well characterized at the molecular level (DP O'Keefe, KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak, H Yu, R Sirevag, RE Christoffersen [1990] Proc Natl Acad Sci USA 87: 3904-3908). Despite this extensive characterization, the role of the enzyme in vivo was not established. Optical and electron paramagnetic resonance binding studies described here suggest that the monoterpenoids, nerol and geraniol, are substrates of avocado cytochrome P-450 (spectral dissociation constant of 7.2 and 35 micromolar, respectively). Avocado microsomes have been shown to catalyze the hydroxylation of these monoterpenoids, and both nerol and geraniol have been shown to inhibit the activity of avocado cytochrome P-450 toward the artificial substrate 7-ethoxycoumarin, with nerol a competitive inhibitor of this activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62b9da4a2f6e8107f1fd18403db409f6 https://doi.org/10.1104/pp.98.4.1290 Zobrazit plný text záznamu