Preparation of high-affinity rabbit monoclonal antibodies for ciprofloxacin and development of an indirect competitive ELISA for residues in milk
| Autor: | Bin Huang, Lei Lu, Hai Ding, Yun Yin, Ting Yu, Xiaodong Zheng, Yan-zhen Zhang, Jia-jin Zhu, Lin Wang |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Fleroxacin medicine.drug_class Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody General Biochemistry Genetics and Molecular Biology Pefloxacin Antibody Specificity Ciprofloxacin medicine Enrofloxacin Animals General Pharmacology Toxicology and Pharmaceutics Norfloxacin Detection limit Chromatography General Veterinary Chemistry Antibodies Monoclonal General Medicine Drug Residues Anti-Bacterial Agents Milk Cattle Rabbits Ofloxacin Haptens Biotechnology medicine.drug |
| Zdroj: | Journal of Zhejiang University SCIENCE B. 11:812-818 |
| ISSN: | 1862-1783 1673-1581 |
| DOI: | 10.1631/jzus.b1000055 |
| Popis: | A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin (CPFX) was developed by using rabbit monoclonal antibodies (RabMAbs) against a hapten-protein conjugate of CPFX-bovine serum albumin (BSA). The indirect competitive ELISA of CPFX had a concentration at 50% inhibition (IC(50)) of 1.47 ng/ml and a limit of detection (LOD) of 0.095 ng/ml. The mAb exhibited some cross-reactivity, however, not so high with enrofloxacin (28.8%), ofloxacin (13.1%), norfloxacin (11.0%), fleroxacin (22.6%), and pefloxacin (20.4%). And it showed almost no cross-reactivity with other antibiotics or sulfonamides evaluated in this study. The competitive ELISA kit developed here could be used as a screening tool to detect and control illegal addition of CPFX in food products. This kit had been applied to milk detection and the recovery rates from samples spiked by CPFX were in a range of 63.02%-84.60%, with coefficients of variation of less than 12.2%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink