Interleukin-10 rs1800871 (−819C/T) and ATA haplotype are associated with preeclampsia in a Tunisian population
| Autor: | Hedia Zitouni, Marwa Ben Ali Gannoun, Nozha Raguema, S. Meddeb, Julie L. Lavoie, Dhafer Benletaifa, Wassim Y. Almawi, Touhami Mahjoub |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
medicine.medical_specialty Linkage disequilibrium Tunisia Blood Pressure Polymorphism Single Nucleotide Linkage Disequilibrium Preeclampsia Young Adult 03 medical and health sciences 0302 clinical medicine Gene Frequency Pre-Eclampsia Pregnancy Risk Factors Internal medicine Genotype Odds Ratio Internal Medicine medicine Genetic predisposition Humans Genetic Predisposition to Disease Promoter Regions Genetic Genetic Association Studies Retrospective Studies Chi-Square Distribution 030219 obstetrics & reproductive medicine business.industry Haplotype Obstetrics and Gynecology Odds ratio medicine.disease Interleukin-10 Logistic Models Phenotype Haplotypes Multivariate Analysis Cohort Female business 030215 immunology Cohort study |
| Zdroj: | Pregnancy Hypertension. 11:105-110 |
| ISSN: | 2210-7789 |
| DOI: | 10.1016/j.preghy.2018.01.011 |
| Popis: | Objectives Interleukin-10 (IL-10) is implicated in several aspects of pregnancy. As genetic predisposition can be involved in the development of preeclampsia, the association between IL-10’s promoter region polymorphisms and this pathology has been investigated, although with conflicting results. To date, only a small cohort study (total n = 40) has evaluated this association in the African continent, and none have been conducted in Tunisia. Hence, we evaluated the association between these polymorphisms and the risk of preeclampsia in a large Tunisian cohort. Study design 345 preeclampsia patients and 300 controls were genotyped for the IL-10 promoter region variants −1082A/G, −819C/T and −592A/C using real-time PCR. Main outcome measures Differences in means were determined by Student’s t-test, while intergroup significance was assessed by Pearson χ2 or 2-way ANOVA. Genotypes were tested for Hardy–Weinberg equilibrium (HWE) in the control and cases. Logistic regression analysis was performed in order to determine the odds ratios and 95% confidence intervals associated with the linkage disequilibrium risk. Results An increased frequency of the −819 T (minor) allele and the −819 T/T genotype was seen in preeclampsia cases. Also, three-locus haplotype (−1082AG/−819CT/−592AC) analysis identified the ATA haplotype as having a higher incidence in women with preeclampsia (OR = 1.48, 95% CI: 1.03–2.11) and this was confirmed by multivariate regression analysis (OR = 1.65, 95% CI: 1.13–2.43) after controlling for covariates. Conclusions We suggest that the IL-10 −819 T/T variant and the ATA haplotype, which are associated with low production of IL 10, represent genetic risk factors for preeclampsia in Tunisian women. |
| Databáze: | OpenAIRE |
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