Cloning of a SEPALLATA4-like gene (IiSEP4) in Isatis indigotica Fortune and characterization of its function in Arabidopsis thaliana
| Autor: | Yan-Qin Ma, Zi-Qin Xu, Meng-Xin Lu, Zuo-Qian Pu, Ye-Ye Ma |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine animal structures Physiology Arabidopsis Stamen MADS Domain Proteins Flowers Plant Science 01 natural sciences Sepal 03 medical and health sciences Gene Expression Regulation Plant Genetics Arabidopsis thaliana Cloning Molecular Isatis Ovule Gene Phylogeny Plant Proteins Cloning biology fungi food and beverages Plants Genetically Modified biology.organism_classification 030104 developmental biology Inflorescence Function (biology) 010606 plant biology & botany |
| Zdroj: | Plant Physiology and Biochemistry. 154:229-237 |
| ISSN: | 0981-9428 |
| DOI: | 10.1016/j.plaphy.2020.05.031 |
| Popis: | E-class MADS-box genes, SEPALLATA (SEP), participate in various aspects of plant development together with B-, C- and D-class MADS-box genes. IiSEP4, a homologous gene of SEP4, was cloned from Isatis indigotica. IiSEP4 was highly expressed in sepals, and its mRNA was mildly detected in leaves, inflorescences, flowers, stamens and young silicles. Constitutive expression of IiSEP4 in Arabidopsis thaliana caused early flowering, accompanied by the reduction of flowers and floral organs. Moreover, the sepals in some flowers were transformed into carpelloid structures with stigmatic papillae, and obviously accompanied by ovule formation. Yeast two-hybrid assays demonstrated that IiSEP4 interacts with other woad MADS proteins to determine the identity of floral organs. These findings reveal the important roles of IiSEP4 in floral development of I. indigotica. The results of this study can lay a foundation for further study on biological functions of MADS transcriptional factors in I. indigotica. |
| Databáze: | OpenAIRE |
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