Tubulation of Supported Lipid Bilayer Membranes Induced by Photosensitized Lipid Oxidation
| Autor: | Luke R. Jordan, Ashley M. Baxter, Nathan J. Wittenberg, Monicka Kullappan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Fluorophore
Chemistry Vesicle Phospholipid 02 engineering and technology Surfaces and Interfaces Radius 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences 0104 chemical sciences Light intensity chemistry.chemical_compound Membrane Lipid oxidation Electrochemistry Biophysics lipids (amino acids peptides and proteins) General Materials Science 0210 nano-technology Lipid bilayer Spectroscopy |
| Zdroj: | Langmuir. 37:5753-5762 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | We show that photosensitized phospholipid oxidation, initiated by the lipid-conjugated fluorophore TopFluor-PC, causes defects, namely, membrane tubes and vesicle-like structures, in supported lipid bilayers (SLBs). Lipid oxidation is detrimental to the integrity of the lipid molecules; when oxidized, they undergo a conformational expansion, which causes membrane tubes to protrude from the SLB. Lipid oxidation is verified by FT-IR spectroscopy, and area expansion is observed in Langmuir trough experiments. Upon growing to a critical length, the membrane tubes arising from SLBs rapidly undergo transition to vesicle-like structures. We find a correlation between the maximum tube length and the diameter of the resulting vesicle, suggesting the conservation of the surface area between these features. We use geometric modeling and the measured tube length and vesicle radius to calculate the tube radius; our calculated mean tube diameter of 243 nm is comparable to other groups' experimental findings. In the presence of fluid flow, membrane tubes can be extended to tens to hundreds of microns in length. SLBs composed of saturated lipids resist light-induced tubulation, and the inclusion of the lipophilic antioxidant α-tocopherol attenuates the tubulation process and increases the light intensity threshold for tubulation. |
| Databáze: | OpenAIRE |
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