Apoptosis of Chondrocytes in Transgenic Mice Lacking Collagen II
| Autor: | Jaspal S. Khillan, Yunhua Bao, Darwin J. Prockop, Helminen Hj, Chunlin Yang, Shi-Wu Li |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cartilage
Articular Genetically modified mouse Cartilage Connective tissue Apoptosis Mice Transgenic Cell Biology Osteoarthritis Biology medicine.disease Extracellular Matrix Cell biology Extracellular matrix Mice Collagen type I alpha 1 medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Immunology medicine Animals Collagen Fragmentation (cell biology) |
| Zdroj: | Experimental Cell Research. 235:370-373 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.1997.3692 |
| Popis: | Previous work demonstrated that collagen fibrils were not detectable in the cartilage of transgenic mice homozygous for targeted inactivation of the collagen II gene. In the present work, we used the same mice to show that chondrocytes undergo apoptosis in the absence of collagen II, the major component of the extracellular matrix of cartilage. The chondrocytes in the homozygous mice had condensed nuclei, fragmentation of nuclear DNA, and decreased levels of the Bcl-2 protein. These results provide direct evidence that cartilage extracellular matrix lacking collagen II cannot support the survival of chondrocytes. In addition, the results suggest that apoptosis may play a role in degenerative connective tissue diseases such as osteoarthritis in which there is extensive tissue loss. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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