Level of double negative T cells, which produce TGF-β and IL-10, predicts CD8 T-cell activation in primary HIV-1 infection
| Autor: | Laurence Weiss, Michaela Müller-Trutwin, Maria Elena Manea, Céline Didier, Gaël Petitjean, Anne-Sophie Liovat, Daniel Scott-Algara, Feriel Tibaoui, Pierre-Marie Girard, Laurence Meyer, Françoise Barré-Sinoussi, Mathieu F. Chevalier, Pauline Campa |
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| Přispěvatelé: | Régulation des Infections Rétrovirales, Institut Pasteur [Paris] (IP), Université Paris Diderot - Paris 7 (UPD7), Centre de recherche en épidémiologie et santé des populations (CESP), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Université Paris-Sud - Paris 11 (UP11)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université Paris Descartes - Paris 5 (UPD5), This work was supported by the ANRS and the Assistance Publique – Hôpitaux de Paris (AP-HP, Paris)., Institut Pasteur [Paris], Hôpital Paul Brousse-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), CHU Saint-Antoine [APHP] |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male medicine.medical_treatment HIV Infections CD38 CD8-Positive T-Lymphocytes Lymphocyte Activation T-Lymphocytes Regulatory MESH: HIV Infections/pathology MESH: Lymphocyte Activation*/genetics 0302 clinical medicine Transforming Growth Factor beta Immunology and Allergy Cytotoxic T cell MESH: HIV Infections/genetics IL-2 receptor Longitudinal Studies Prospective Studies MESH: Longitudinal Studies 0303 health sciences MESH: T-Lymphocytes Regulatory/immunology Chemistry FOXP3 Forkhead Transcription Factors 3. Good health Interleukin-10 Interleukin 10 Infectious Diseases Cytokine MESH: CD8-Positive T-Lymphocytes/immunology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Disease Progression [SDV.IMM]Life Sciences [q-bio]/Immunology Female MESH: Disease Progression France MESH: Immunophenotyping Immunology MESH: Forkhead Transcription Factors/immunology Immunophenotyping MESH: CD4-Positive T-Lymphocytes/immunology 03 medical and health sciences medicine Humans MESH: Interleukin-10/metabolism Interleukin-7 receptor 030304 developmental biology MESH: HIV-1/immunology MESH: Humans MESH: HIV Infections/immunology MESH: Male MESH: Prospective Studies MESH: France HIV-1 MESH: Female CD8 MESH: Transforming Growth Factor beta/metabolism 030215 immunology |
| Zdroj: | AIDS. Official journal of the international AIDS Society AIDS. Official journal of the international AIDS Society, 2012, 26 (2), pp.139-148. ⟨10.1097/QAD.0b013e32834e1484⟩ AIDS AIDS, Lippincott, Williams & Wilkins, 2012, 26 (2), pp.139-148. ⟨10.1097/QAD.0b013e32834e1484⟩ |
| ISSN: | 0269-9370 1473-5571 |
| DOI: | 10.1097/QAD.0b013e32834e1484⟩ |
| Popis: | International audience; OBJECTIVE:Persistent immune activation plays a central role in the pathogenesis of HIV disease. Besides natural regulatory T cells (nTregs), 'double negative' T cells shown to exhibit regulatory properties could be involved in the control of harmful immune activation. The aim of this study was to analyze, in patients with primary HIV infection (PHI), the relationship between CD4(+)CD25(+)CD127(low)FoxP3(+) nTregs or CD3(+)CD4(-)CD8(-) double negative T cells and systemic immune activation.DESIGN:A prospective longitudinal study of patients with early PHI.METHODS:Twenty-five patients were included. Relationships between frequency of Treg subsets and T-cell activation, assessed on fresh peripheral blood mononuclear cells, were analyzed using nonparametric tests. Cytokine production by double negative T cells was assessed following anti-CD3/anti-CD28 stimulation.RESULTS:No relationship was found between T-cell activation and frequencies of nTregs. In contrast, a strong negative relationship was found at baseline between the proportion of double negative T cells and the proportion of activated CD8 T cells coexpressing CD38 and HLA-DR (P = 0.005) or expressing Ki-67 (P = 0.002). In addition, the frequency of double negative T cells at baseline negatively correlated with the frequency of HLA-DR(+)CD38(+)CD8(+) T cells at month 6, defining the immune activation set point (P = 0.031). High proportions of stimulated double negative T cells were found to produce the immunosuppressive cytokines transforming growth factor-β1 and/or IL-10.CONCLUSION:The proportion of double negative T cells at baseline was found to be predictive of the immune activation set point. Our data strongly suggest that double negative T cells may control immune activation in PHI. This effect might be mediated through the production of TGF-β1/IL-10 known to downmodulate immune activation. |
| Databáze: | OpenAIRE |
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