Analysis of diluted vitreous samples from vitrectomy is useful in eyes with severe acute postoperative endophthalmitis
| Autor: | Chiquet , Christophe, Maurin , Max, Thuret , Gilles, Benito , Yvonne, Cornut , Pierre-Loïc, Creuzot-Garcher , Catherine, Rouberol , Frédéric, Pechinot , André, Lina , Gérard, Romanet , Jean-Paul, Bron , Alain, Vandenesch , François, Renseigné , Non |
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| Přispěvatelé: | Department of Ophtalmology, CHU Grenoble, Département des agents infectieux, Service d'ophtalmologie, Université Jean Monnet [Saint-Étienne] ( UJM ) -Hôpital Bellevue, Immunité infection vaccination ( I2V ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-IFR128-Institut National de la Santé et de la Recherche Médicale ( INSERM ), Laboratoire de Bactériologie, Hospices Civils de Lyon ( HCL ), Hôpital Edouard Herriot [CHU - HCL], Hospices Civils de Lyon ( HCL ) -Hospices Civils de Lyon ( HCL ), Service d'Ophtalmologie (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand ( CHU Dijon ), Laboratoire de Microbiologie Médicale et Moléculaire, Université de Bourgogne ( UB ), Université Jean Monnet [Saint-Étienne] (UJM)-Hôpital Bellevue, Immunité infection vaccination (I2V), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR128-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), Université de Bourgogne (UB) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
genetic structures medicine.medical_treatment Colony Count Microbial MESH : Aged MESH : Prospective Studies MESH : Vitrectomy Vitrectomy [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology Polymerase Chain Reaction Eye Infections Bacterial Cohort Studies Postoperative Complications 0302 clinical medicine Endophthalmitis RNA Ribosomal 16S MESH: Postoperative Complications MESH : DNA Ribosomal MESH : DNA Bacterial MESH : Female Prospective Studies MESH: Cohort Studies MESH : Polymerase Chain Reaction MESH: Aged MESH: Cataract Extraction 0303 health sciences MESH: DNA Ribosomal MESH: Eye Infections Bacterial MESH : Acute Disease Outcome measures Diagnostic test Bacterial Typing Techniques 3. Good health MESH: RNA Ribosomal 16S medicine.anatomical_structure Acute Disease MESH: Acute Disease MESH : Eye Infections Bacterial Female DNA Bacterial Pars plana medicine.medical_specialty MESH : Cataract Extraction MESH : Colony Count Microbial MESH : Male MESH : Endophthalmitis MESH : Cohort Studies Cataract Extraction DNA Ribosomal MESH: Bacterial Typing Techniques 03 medical and health sciences McNemar's test Ophthalmology medicine Humans MESH: Endophthalmitis MESH : Bacteria MESH: Colony Count Microbial Aged Brain-heart Infusion broth MESH: Humans Bacteria 030306 microbiology business.industry MESH: Vitreous Body MESH : Humans MESH: Polymerase Chain Reaction medicine.disease MESH: DNA Bacterial [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology MESH: Male MESH: Prospective Studies eye diseases Vitreous Body MESH : RNA Ribosomal 16S MESH: Bacteria MESH: Vitrectomy 030221 ophthalmology & optometry MESH : Bacterial Typing Techniques MESH : Postoperative Complications business MESH : Vitreous Body MESH: Female Acute postoperative endophthalmitis |
| Zdroj: | Ophthalmology Ophthalmology, 2009, 116 (12), pp.2437-41.e1. 〈10.1016/j.ophtha.2009.06.007〉 Ophthalmology: Journal of The American Academy of Ophthalmology Ophthalmology: Journal of The American Academy of Ophthalmology, Elsevier, 2009, 116 (12), pp.2437-41.e1. ⟨10.1016/j.ophtha.2009.06.007⟩ |
| ISSN: | 0161-6420 |
| DOI: | 10.1016/j.ophtha.2009.06.007〉 |
| Popis: | Purpose This study was designed to compare the diagnostic yield of microbiological analysis performed on diluted and undiluted vitreous samples from pars plana vitrectomy (PPV) in patients with acute postcataract endophthalmitis. Design Cohort study, evaluation of diagnostic test or technology. Participants Patients with acute postcataract endophthalmitis ( Methods Undiluted and diluted vitreous samples were taken from 34 consecutive patients at the beginning of PPV as part of the multicenter prospective study of the French Institutional Endophthalmitis Study (FRIENDS) group. Vitrectomy was performed after 1 (n = 12) or 2 (n = 22) intravitreous antibiotic injections. McNemar's nonparametric test was used to compare culture and polymerase chain reaction (PCR) results between diluted and undiluted samples. Main Outcome Measures Rate of positivity of conventional culture (brain heart infusion broth) and eubacterial PCR tests from undiluted and diluted vitreous samples. Results The microbiological analysis of both undiluted and diluted vitreous samples detected and identified a bacterial pathogen in 26 out of 34 cases (76.4%). The analysis of undiluted and diluted vitreous at the time of PPV, using eubacterial PCR and conventional culture, gave similar results ( P = 0.99; McNemar test). However, eubacterial PCR was more sensitive than culture in detecting bacteria in vitreous at the time of PPV (76% vs 6%; P = 0.001; McNemar test). The difference in sensitivity between the 2 techniques was primarily associated with false-negative culture results for undiluted samples (2/3 of cases), mainly for coagulase-negative staphylococci. Conclusions The microbiological results obtained combining PCR and culture techniques were similar for diluted vitreous and undiluted vitreous analysis. When eubacterial PCR is available, sampling diluted vitreous, an easier procedure, may replace sampling undiluted vitreous. Financial Disclosure(s) The authors have no proprietary or commercial interest in any of the materials discussed in this article. |
| Databáze: | OpenAIRE |
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