Interactions among the sevenHelicobacter pyloriproteins encoded by the urease gene cluster
| Autor: | David L. Weeks, Petra Voland, Elizabeth A. Marcus, George Sachs, Christian Prinz, David R. Scott |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Electrophoresis
Gram-negative bacteria Urease Physiology Spirillaceae Two-hybrid screening In Vitro Techniques Microbiology Gastric Acid Bacterial Proteins Two-Hybrid System Techniques Physiology (medical) chemistry.chemical_classification Helicobacter pylori Hepatology biology Membrane transport protein Cell Membrane Gastroenterology Membrane Transport Proteins Phosphate-Binding Proteins biology.organism_classification Precipitin Tests Enzyme Biochemistry chemistry Mutagenesis biology.protein Carrier Proteins Bacteria |
| Zdroj: | American Journal of Physiology-Gastrointestinal and Liver Physiology. 284:G96-G106 |
| ISSN: | 1522-1547 0193-1857 |
| DOI: | 10.1152/ajpgi.00160.2002 |
| Popis: | Survival of Helicobacter pylori in acid depends on intrabacterial urease. This urease is a Ni2+-containing oligomeric heterodimer. Regulation of its activity and assembly is important for gastric habitation by this neutralophile. The gene complex encodes catalytic subunits ( ureA/B), an acid-gated urea channel ( ureI), and accessory assembly proteins ( ureE–H). With the use of yeast two-hybrid analysis for determining protein-protein interactions, UreF as bait identified four interacting sequences encoding UreH, whereas UreG as bait detected five UreE sequences. These results were confirmed by coimmunoprecipitation and β-galactosidase assays. Native PAGE immunoblotting of H. pylori inner membranes showed interaction of UreA/B with UreI, whereas UreI deletion mutants lacked this protein interaction. Deletion of ureE–H did not affect this interaction with UreI. Hence, the accessory proteins UreE/G and UreF/H form dimeric complexes and UreA/B form a membrane complex with UreI, perhaps enabling assembly of the urease apoenzyme at the membrane surface and immediate urea access to intrabacterial urease to allow rapid periplasmic neutralization. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|