Severe acute respiratory syndrome vaccine efficacy in ferrets: whole killed virus and adenovirus-vectored vaccines
| Autor: | Karuna P. Karunakaran, Rachel L. Roper, Jack Gauldie, Martin Petric, Thomas Rowe, Thomas G. Voss, Lois A. Zitzow, Robert C. Brunham, B. Brett Finlay, David Lawrence, Catherine P. Y. Mok, Raymond H. See |
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| Rok vydání: | 2008 |
| Předmět: |
Male
viruses Genetic Vectors Antibodies Viral Severe Acute Respiratory Syndrome Vaccines Attenuated Virus Neutralization Tests Virology medicine Animals Humans Vector (molecular biology) skin and connective tissue diseases Neutralizing antibody Lung Vaccines Synthetic biology Adenoviruses Human fungi Respiratory disease Ferrets Viral Vaccines medicine.disease Vaccine efficacy body regions Disease Models Animal Pneumonia medicine.anatomical_structure Severe acute respiratory syndrome-related coronavirus Vaccines Inactivated Immunology biology.protein Viral disease Respiratory tract |
| Zdroj: | Journal of General Virology. 89:2136-2146 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/vir.0.2008/001891-0 |
| Popis: | Although the 2003 severe acute respiratory syndrome (SARS) outbreak was controlled, repeated transmission of SARS coronavirus (CoV) over several years makes the development of a SARS vaccine desirable. We performed a comparative evaluation of two SARS vaccines for their ability to protect against live SARS-CoV intranasal challenge in ferrets. Both the whole killed SARS-CoV vaccine (with and without alum) and adenovirus-based vectors encoding the nucleocapsid (N) and spike (S) protein induced neutralizing antibody responses and reduced viral replication and shedding in the upper respiratory tract and progression of virus to the lower respiratory tract. The vaccines also diminished haemorrhage in the thymus and reduced the severity and extent of pneumonia and damage to lung epithelium. However, despite high neutralizing antibody titres, protection was incomplete for all vaccine preparations and administration routes. Our data suggest that a combination of vaccine strategies may be required for effective protection from this pathogen. The ferret may be a good model for SARS-CoV infection because it is the only model that replicates the fever seen in human patients, as well as replicating other SARS disease features including infection by the respiratory route, clinical signs, viral replication in upper and lower respiratory tract and lung damage. |
| Databáze: | OpenAIRE |
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