A novel member of the subtilisin-like protease family from Bacillus subtilis
| Autor: | Alessandra M. Albertini, Eugenio Ferrari, Angela Valbuzzi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Gene Expression Bacillus subtilis Polymerase Chain Reaction Microbiology Primer extension Subtilase Bacterial Proteins Species Specificity Consensus Sequence Amino Acid Sequence RNA Messenger Phylogeny Regulator gene Electrophoresis Agar Gel Serine protease Bacillaceae Base Sequence biology Serine Endopeptidases fungi Subtilisin beta-Galactosidase biology.organism_classification Molecular biology RNA Bacterial Biochemistry Genes Bacterial biology.protein Subtilisins |
| Zdroj: | Microbiology. 145:3121-3127 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-145-11-3121 |
| Popis: | aprX is a 1326 bp gene of Bacillus subtilis strain 168 that encodes a serine protease, probably intracellular, characterized by significant similarity with subtilisins, thermitases and pyrolysins. Transcription analysis, performed by RT-PCR and primer extension, allowed the localization of the active promoter and showed that aprX is expressed in stationary phase. The pattern of expression of aprX and its dependence on various transition state regulatory genes (degU, degQ, hpr, abrB, sinR), monitored by lacZ transcriptional fusions, are distinctive from those of subtilisin and other degradative enzymes. aprX is not essential for either growth or sporulation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|