Suppression of Sox6 in P19 cells leads to failure of neuronal differentiation by retinoic acid and induces retinoic acid-dependent apoptosis
| Autor: | Kyoko Ishikawa, Miyuki Kanai, Masaharu Miyake, Yuichi Kawai, Michiko Hamada-Kanazawa, Daisuke Ogawa, Masanori Narahara |
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| Rok vydání: | 2004 |
| Předmět: |
Neurite
Cellular differentiation Biophysics Retinoic acid Apoptosis Biology Biochemistry Cell Line Mice chemistry.chemical_compound Structural Biology Genetics Animals Molecular Biology Neurons Reverse Transcriptase Polymerase Chain Reaction High Mobility Group Proteins Cell Differentiation Cell Biology Transfection Immunohistochemistry Molecular biology Embryonic stem cell DNA-Binding Proteins P19 cell Neuronal differentiation chemistry embryonic structures DNA fragmentation Embryonal carcinoma P19 Sox6 SOXD Transcription Factors Transcription Factors |
| Zdroj: | FEBS Letters. 577:60-66 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2004.09.063 |
| Popis: | The Sox6 gene is a member of the Sox gene family, which encodes transcription factors, and previous studies have suggested that it plays an important role in the development of the central nervous system. Aggregation of embryonic carcinoma P19 cells with retinoic acid (RA) results in the development of neurons, glia, and fibroblast-like cells. Sox6 mRNA increases rapidly in P19 cells during RA induction and then decreases during differentiation into neuronal cells. To investigate whether Sox6 expression is essential for neuronal differentiation, we established Sox6-suppressed P19 (P19[anti-Sox6]) cells by transfection of antisense-Sox6 cDNA. Most of the P19[anti-Sox6] cells showed no neurites and were not stained by the anti-MAP 2 antibody, while the suppression of Sox6 expression nearly totally blocked neuronal differentiation in P19 cells. Further, Sox6 suppression caused RA-dependent apoptosis by P19[anti-Sox6] cells: RA-treated P19[anti-Sox6] cells showed chromatin condensation, DNA fragmentation, and an increase in caspase-3-like activity. Thus, Sox6 is considered essential for neuronal differentiation and may play an important role in the early stages of neuronal differentiation or apoptosis. |
| Databáze: | OpenAIRE |
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