Overexpression ofCYP19A1aromatase in Leydig cells is associated with steroidogenic dysfunction in subjects with Sertoli cell-only syndrome
| Autor: | María Cecilia Lardone, F. Argandoña, Alexis Parada-Bustamante, Antonio Piottante, M. Flórez, Mauricio Ebensperger, Cristian Palma, Andrea Castro |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine endocrine system medicine.medical_specialty Urology Endocrinology Diabetes and Metabolism Laser Capture Microdissection Andrology Sertoli cell-only syndrome 03 medical and health sciences Aromatase 0302 clinical medicine Endocrinology Internal medicine Testis medicine Humans Testosterone Transcriptional expression Spermatogenesis Laser capture microdissection 030219 obstetrics & reproductive medicine Estradiol biology Sertoli Cell-Only Syndrome Leydig Cells Luteinizing Hormone Hyperplasia medicine.disease 030104 developmental biology Reproductive Medicine biology.protein Follicle Stimulating Hormone Hormone |
| Zdroj: | Andrology. 5:41-48 |
| ISSN: | 2047-2919 |
| Popis: | Summary Several observational studies have showed a combination of lower testosterone (T) to LH ratio and higher estradiol (E2) to T ratio in secretory infertile men compared to men with normal spermatogenesis, suggesting a steroidogenic dysfunction of Leydig cells (Lc) that may involve increased aromatase activity. Low T/LH ratio is associated with Lc hyperplasia, which together with LH hyperstimulation may represent compensation for impaired T production. Aromatase expression and oestrogen production are mainly detected in Lc of the testis, although Sertoli and germ cells also contribute to testicular aromatase activity. The aim of this study was to assess the transcriptional expression of CYP19A1 (aromatase) in isolated Lc of subjects with Sertoli cell-only syndrome (SCOS) and signs of Lc impairment. Nineteen patients with SCOS and 10 controls with normal spermatogenesis who had medical indication of testicular biopsy for sperm retrieval were studied. Leydig cells were isolated by Laser Capture Microdissection (LCM) and CYP19A1 mRNA expression was quantified by SYBR® Green-based qPCR. In addition, testicular T and E2 and serum hormonal levels were measured. Relative to control group, CYP19A1 was overexpressed more than twofold in 10/19 cases (2.3–12.2-fold increase), showing a significant increment in cases with low T/LH ratio (T/LH |
| Databáze: | OpenAIRE |
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